Antigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodies

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dc.contributor.authorLefebvre, D. J.
dc.contributor.authorCosters, S.
dc.contributor.authorDoorsselaere, J. V.
dc.contributor.authorMisinzo, G.
dc.contributor.authorDelputte, P. L.
dc.contributor.authorNauwynck, H. J.
dc.date.accessioned2017-06-28T07:22:28Z
dc.date.available2017-06-28T07:22:28Z
dc.date.issued2008
dc.descriptionJournal of General Virology 2008, Vol. 89:pp 177โ€“187en_US
dc.description.abstractThis study examined whether antigenic differences among porcine circovirus type 2 (PCV-2) strains could be detected using monoclonal antibodies (mAbs). A subtractive immunization protocol was used for the genotype 2 post-weaning multisystemic wasting syndrome (PMWS)-associated PCV-2 strain Stoon-1010. Sixteen stable hybridomas that produced mAbs with an immunoperoxidase monolayer assay (IPMA) titre of 1000 or more to Stoon-1010 were obtained. Staining of recombinant PCV-2 virus-like particles demonstrated that all mAbs were directed against the PCV-2 capsid protein. Cross-reactivity of mAbs was tested by IPMA and neutralization assay for genotype 1 strains 48285, 1206, VC2002 and 1147, and genotype 2 strains 1121 and 1103. Eleven mAbs (9C3, 16G12, 21C12, 38C1, 43E10, 55B1, 63H3, 70A7, 94H8, 103H7 and 114C8) recognized all strains in the IPMA and demonstrated neutralization of Stoon-1010, 48285, 1206 and 1103, but not VC2002, 1147 and 1121. mAbs 31D5, 48B5, 59C6 and 108E8 did not react with genotype 1 strains or had a reduced affinity compared with genotype 2 strains in the IPMA and neutralization assay. mAb 13H4 reacted in the IPMA with PMWS-associated strains Stoon-1010, 48285, 1206 and VC2002, and the porcine dermatitis and nephropathy syndrome-associated strain 1147, but not with reproductive failure-associated strains 1121 and 1103. mAb 13H4 did not neutralize any of the tested strains. It was concluded that, despite the high amino acid identity of the capsid protein (ยข91 %), antigenic differences at the capsid protein level are present among PCV-2 strains with a different genetic and clinical background.en_US
dc.identifier.issn2008
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/1730
dc.language.isoenen_US
dc.publisherJournal of General Virologyen_US
dc.subjectPorcine circovirusen_US
dc.subjectMonoclonal antibodiesen_US
dc.subject2 (PCV-2)en_US
dc.subjectImmunoperoxidase monolayer assayen_US
dc.titleAntigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodiesen_US
dc.typeArticleen_US
dc.urlhttp://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83280-0en_US

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