Species and gender differentiation between and among domestic and wild animals using mitochondrial and sex-linked DNA markers
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Date
2005-11
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Abstract
In many African countries accurate and reliable identification of poached wildlife products like
carcasses or meat presents a big problem when morphological characters such as skin hair or bones
are missing. We describe a molecular based approach that has a potential of serving as a forensic tool
in game meat identification in Africa. A mitochondial DNA marker (mt700) and one restriction enzyme,
Rsa1 were used in the PCR-RFLP species identification of game meat obtained from two National Parks
in Tanzania. Species-specific reference DNA fragment patterns were obtained using fresh meat from ten
wildlife and four domesticated species. All species except the zebra, produced unique monomorphic
RFLP patterns. Collectively, these patterns demonstrate the potential ability of genetic techniques for
discriminating between and among wildlife and domestic species. The reference PCR-RFLP fragments
enabled species identification of about 79% of unknown meat samples. In addition, sex was also
assigned to all of the samples following successful amplification of gender-specific, SRY and ZFY/X,
chromosomal domains. Although the present study has been conducted on a limited range both in
numbers and genetic diversity of wildlife species present in Africa, the results demonstrate the
potential usefulness of the DNA approach in wildlife forensics in the continent.
Description
African Journal of Biotechnology Vol. 4 (11), pp. 1269-1274, November 2005
Keywords
Mitochondrial DNA, Poaching, Forensic, Gender, Species identification, PCR