Molecular detection of arboviruses in yellow fever mosquitoes collected from Kyela district, Tanzania
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Date
2016-10
Journal Title
Journal ISSN
Volume Title
Publisher
ResearchGate
Abstract
Arboviruses belong to a group of viruses that are transmitted by arthropods, mainly mosquitoes
and ticks causing clinical disease symptoms in humans and animals ranging from febrile
illnesses to hemorrhagic fevers. The present study aimed at examining the circulation of
Chikungunya, Dengue, Yellow fever and Rift valley fever viral genomes in Aedes mosquitoes
from Kyela district in Tanzania. A systematic vector surveillance spanning two months and
covering five sites in Kyela district was carried out in order to evaluate the potential role of
Aedes spp. in arbovirus transmission in the study area. Mosquitoes were collected, identified
to species level by using morphological keys, pooled in respect species and collection sites
and screened for arboviruses by RT-PCR. Adult mosquitoes were collected from April to
May, 2015 using CO 2 -baited CDC light traps, magnet traps as well as human landing collection
(HLC). The study sites included Kyela town, Kajunjumele, Ipida, Matema and Njisi villages.
A total of 480 blood-feed Aedes ssp. were collected, identified and grouped into 24 pools (1-
20 mosquitoes per pool) according to species level and location. Out of the 480 Aedes spp.
collected, Aedes aegyptir epresented the most abundant species totaling 338 (70.4%),
followed by Aedes africanus 102 (21.2%) and Aedes natalensis being the minority, 40
(8.3%). Arboviruses were detected in nine pools (37.5%) including Alpha viruses (8 pools)
and Flavi viruses (1 pool). No sample was positive for Bunya viruses.Chikungunya virus
(CHIKV) was detected in 6 (75%) alpha virus positive pools that were collected mostly in
the areas where rice cultivation was common. The findings of this study suggest that
inhabitants in this region are highly likely to be exposed to arbovirus infections which may
represent significant public health concerns.
Description
Keywords
Abundance, Aedes, Kyela district, Molecular diagnosis, Arboviruses