Antibiogram and diversity of extended-spectrum beta-lactamase genes in scavenging local chicken in Morogoro municipality, Tanzania
Loading...
Date
2020-02-20
Journal Title
Journal ISSN
Volume Title
Publisher
Excellent publishers
Abstract
The poultry industry, especially chicken production has in recent times faced a
major set-back due to devastating effects of APEC Extended Spectrum Beta-
Lactases (ESBL) producing organisms. This research aimed at investigating
antimicrobial susceptibility profile and diversity of ESBL producing avian
pathogenic Escherichia coli(APEC) among fecal cloacal swaps of scavenging
local chickens based on the various housing systems. The APEC isolates were
determined by virulence factor profiling and by Kirby-Baeur disc diffusion, 42%
of the APEC isolates were found to be ESBL producers. Of the ESBL isolates,
87.5% were resistant to nalidixc acid, 37.5% were resistant to cefotaxime,
Trimethroprim-Sulfamethoxazole, augmentnin
and cephalothin, 25% were
resistant to Ceftriaxone whiles no isolate was resistant to Imipenem,
gentamycin and ciprofloxacin. On screening, a total of 32 beta-lactamase genes
were found amongst these isolates, all of these isolates harbored the blaTEM
gene.Semi-intensively kept chickens harbored more ESBL genes and in more
diverse forms than the extensively kept ones.
Description
Keywords
Antibiogram, Extended-Spectrum Beta-Lactamase, Scavenging, Avian pathogenic Escherichia coli