Bioactive compounds in Diospyros mafiensis roots inhibit growth, sporulation and aflatoxin production by Aspergillus flavus and Aspergillus parasiticus

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dc.contributor.authorMmongoyo, J.A.
dc.contributor.authorNair, M.G.
dc.contributor.authorLinz, J.E.
dc.contributor.authorWu, F.
dc.contributor.authorMugula, J.K.
dc.contributor.authorDissanayake, A.A.
dc.contributor.authorZhang, C.
dc.contributor.authorDay, D.M.
dc.contributor.authorWee, J.M.
dc.contributor.authorStrasburg, G.M.
dc.date.accessioned2024-07-08T08:27:15Z
dc.date.available2024-07-08T08:27:15Z
dc.date.issued2017-01-20
dc.descriptionResearch article
dc.description.abstractDiospyros mafiensis F. White is a medicinal shrub or small tree (6 m tall) widely distributed in the Zanzibar- Inhambane regional mosaic and traditionally used to treat leprosy, diarrhoea, and skin fungal infections in Tanzania and Mozambique. Our objective was to determine the anti-aflatoxigenic properties of compounds from D. mafiensis root bark against vegetative growth, sporulation and aflatoxin production by Aspergillus flavus and Aspergillus parasiticus. Bioassay-guided extraction, fractionation, and isolation of bioactive compounds using A. parasiticus B62 were employed. The bioactive compounds were elucidated using 1 H and 13 CNMR and LC-MS. Growth inhibition was determined by measuring the colony diameter of A. flavus AF3357 and A. parasiticus SU-1 ATCC56775. Inhibitory effects on sporulation were estimated using a haemocytometer. Total aflatoxin was quantified by direct competitive enzyme-linked immunosorbent assay (ELISA). Bioactive compounds diosquinone (DQ) and 3-hydroxydiosquinone (3HDQ) were identified. DQ weakly inhibited A. flavus and A. parasiticus vegetative growth (MIC 50 >100 μg/ml) and 3HDQ strongly inhibited A. flavus (MIC 50 = 14.9 μg/ml) and A. parasiticus (MIC 50 = 39.1 μg/ml). DQ strongly reduced total aflatoxin production by A. flavus from 157 to 36 ng/plate, and by A. parasiticus from 1,145 ng/plate to 45 ng/plate at 100 μg/ml. 3HDQ reduced total aflatoxin production by A. parasiticus from 1,145 to 32 ng/plate; stimulated production by A. flavus from 157 to 872 ng/plate at 12.5 μg/ml but reduced to 45 ng/plate at 100 μg/ ml. In summary, DQ and 3HDQ could be used as natural antifungal compounds to prevent mould growth and aflatoxin accumulation in food and feed.
dc.description.sponsorshipUnited States Agency for International Development (USAID)
dc.identifier.issn1875-0710
dc.identifier.uriDOI 10.3920/WMJ2016.2107
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/6291
dc.language.isoen
dc.publisherWageningen Academic Publishers
dc.relation.ispartofseriesWorld Mycotoxin Journal, 2017; 10 (3): 237-248
dc.subjectDiosquinone
dc.subject3-hydroxydiosquinone
dc.subjectTotal aflatoxin
dc.subjectA. flavus
dc.subjectA. parasiticus
dc.titleBioactive compounds in Diospyros mafiensis roots inhibit growth, sporulation and aflatoxin production by Aspergillus flavus and Aspergillus parasiticus
dc.typeArticle

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