Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomallysosomal system acidification
| dc.contributor.author | Misinzo, G. | |
| dc.contributor.author | Delputte, P. L. | |
| dc.contributor.author | Lefebvre, D. J. | |
| dc.contributor.author | Nauwynck, H. J. | |
| dc.date.accessioned | 2017-06-23T06:28:22Z | |
| dc.date.available | 2017-06-23T06:28:22Z | |
| dc.date.issued | 2007-12-17 | |
| dc.description | Archives virology 2008, Vol. 153: 337–342 | en_US |
| dc.description.abstract | Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-g) or endosomallysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 39% increase), IFN-g (446 8%), ammonium chlorideþ IFN-g (1721 283%), chloroquine diphosphate (1037 121%), chloroquine diphosphateþIFN-g (2199 255%), monensin (950 178%) and monensinþIFN-g (1948 60%). Combined IFNg and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines. | en_US |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/1671 | |
| dc.language.iso | en | en_US |
| dc.subject | Porcine circovirus-2 | en_US |
| dc.subject | PK-15 cells | en_US |
| dc.subject | Endosomallysosomal system | en_US |
| dc.subject | Interferon-gamma (IFN-g) | en_US |
| dc.title | Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomallysosomal system acidification | en_US |
| dc.type | Article | en_US |