Effect of growth regulators on in vivo shoot proliferation and rooting selected local banana (Musa spp.L.) cultivars
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Date
2014
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Sokoine University of Agriculture
Abstract
Dipping banana corms in BAP at 1.5 mg /litre for 12 hours enhances banana sucker
proliferation. Thidiazuron (TDZ) is a growth regulator with both cytokinin and auxin
effect and has a higher residual effect than BAP. The influence of TDZ on shoot
proliferation and root formation in banana is hardly known. The objectives of this study
were to evaluate the effect of (1) in vivo propagation combined with temporary TDZ
treatments on sucker multiplication and growth and (2) indole-3-butyric acid on rooting
and growth of TDZ in vivo derived suckers of banana. The study was conducted at
Sokoine University of Agriculture and comprised of two experiments established in a split
plot arrangement in a randomized complete block design (RCBD). The main factor for
both experiments was banana cultivars (Mtwike, Mzuzu and Bukoba). The sub-factor for
experiment one was TDZ concentrations (0.5, 1.0. 2.0 and 3.0 mg/1) while the sub-factor
for experiment two was 1BA at 0.06 % and control (no 1BA). Data were subjected to
analysis of variance (ANOVA) and mean separation was done based on Student-Newman-
Keuls at P < 5% using GenStat Statistical Programme I2lh Edition. Results showed that
TDZ at 2.0 mg/1 led to the highest proliferation of 6.3 suckers per conn followed by TDZ
at 3.0 and 1.0 mg/1 with 4.6 and 4.0 suckers per corm, respectively. Banana cv. Bukoba
produced the highest number of 6.4 suckers per corm followed by cv. Mzuzu and cv.
Mtwike with 2.9 and 2.3 suckers per corm, respectively. Treatments of TDZ-derived
suckers in IBA powder at 0.06 % reduced root formation and growth. Further studies are
required to determine alternative cytokine-based growth regulators for enhancing banana
shoot productivity under in vivo multiplication
Description
Dissertation
Keywords
Growth regulators, Local banana, Thidiazuron (TDZ), Dipping banana corms, Banana sucker proliferation