Immunohistochemical characterization and quantification of lymphocytes infiltrating lungs during east coast fever.
| dc.contributor.author | Kessy,Vallery Msafiri John | |
| dc.date.accessioned | 2025-10-02T11:24:53Z | |
| dc.date.available | 2025-10-02T11:24:53Z | |
| dc.date.issued | 2002 | |
| dc.description | Dissertation | |
| dc.description.abstract | A study was conducted to investigate the hypothesis that the lungs in East Coast fever are infiltrated by different subsets of lymphocytes that may be responsible in the pathogenesis of pulmonary edema. Four steers 7-10 months old were experimentally infected by subcutaneous injection with 0.5 ml of live Theileria parva sporozoites (Muguga stabilate 3087). Two others remained as uninfected controls. All six animals were clinically monitored daily before and after infection by screening peripheral blood smears, lymph node smears, rectal temperatures, lymph node enlargement, coughing and dyspnea. Also blood samples were taken at intervals of 3-4 days and analysed for total RBC and WBC counts, Hb, PCV and exsanguinated to death under general anaesthesia at the onset of dyspnea, together with one of the controls for sampling of lung tissues. In addition, two lung tissue samples were collected from two ECF naturally infected cattie that were slaughtered during the advanced stage of the disease. The lung tissue samples from the experimentally and naturally infected animals were fixed in 4% neutralbuffered formaldehyde (pH 7.4) for 48 hours and subjected to routine tissue processing procedures to obtain 4 gm thin sections that were stained routinely by H & E for histopathological examination and immunohistochemically by monoclonal antibodies labelled by streptavidin-biotin peroxidase complex to visualize lymphocyte subsets BoCD21+, BoCD4+, BoCD8+ and BoWCl+. Rectal temperatures differential leukocyte count. Each of the infected animal was humanely started to increase above normal by day 8 post infection while ECF was confirmed by lymph node smear examination by day 7 and dyspnea by day 15 post infection. It was demonstrated that the majority of lymphocyte subsets infiltrating the lungs of cattle infected with Theileria parva are BoCD4+ and BoCD8+ in the acute and advanced stages of ECF, respectively. The BoCD8+ T cells were about eleven times more than the rest of the other subsets in the naturally infected animals slaughtered in the advanced stages of ECF. The apparent higher mean number of both BoCD21+ and BoWCl+ during midway of the course of infection suggests that these may have more roles to play in this stage than in the advanced stages of Theileria parva infection. Notwithstanding the mechanisms that attract these lymphocytes into the lungs, their presence indicates that they likely bring about local release of cytokines that contribute towards development of inflammation and pulmonary edema. It was concluded that any endeavour to develop ways to treat Theileria parva infection should study the specific cytokines released during ECF | |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/7070 | |
| dc.language.iso | en | |
| dc.publisher | Sokoine University of Agriculture | |
| dc.subject | Immunohistochemical | |
| dc.subject | Immunohistochemical | |
| dc.subject | Lungs | |
| dc.subject | Pulmonary edema | |
| dc.title | Immunohistochemical characterization and quantification of lymphocytes infiltrating lungs during east coast fever. | |
| dc.type | Thesis |