Typing and characterization of foot-and-mouth disease virus detected in northern part of Tanzania

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dc.contributor.authorMokgele, Kelebogile Portia Oratile
dc.date.accessioned2026-04-18T15:52:19Z
dc.date.available2026-04-18T15:52:19Z
dc.date.issued2014
dc.descriptionMasters Theses
dc.description.abstractFoot-and-mouth disease (FMD) is the most economically important viral disease of livestock globally and has been endemic in Tanzania for many years. It is caused by FMD virus (FMDV). The present study was carried out to identify and differentiate serotypes of the virus from FMD outbreaks in the Northern parts of Tanzania. A total of 35 samples (n = 28 cattle epithelial tissues and n = 7 buffalo probang) were tested by reverseĀ­ transcription polymerase chain reaction (RT-PCR) to detect viral genome, and 86% of the samples (n = 30) typed by antigen detection ELISA. RT-PCR using serotype specific primers targeting the VP1 region of the FMDV genome detected two serotypes (A, and SAT 2), whereas all four serotypes (A, O, SAT 1 and SAT 2) were detected by antigen detection ELISA (Ag-ELISA). Out of the 35 samples, RT-PCR detected 12 positives (34.3%) and of the 12 positive samples, 5(41.7%) were SAT2 and 4 were serotype A (33.3%), and the other 3(25%) have multiple infections. For Ag-ELISA, 20 out of the 30 samples contained FMDV antigen; 3(15%) were SAT1, 4(20%) SAT2, 2(10%) serotype A, and 1(5%) serotype O. The other 10 had multiple infections. Indeed FMD has been confirmed in Serengeti (SAT2, SAT1, and type A), Simanjiro (SAT1, SAT2, type O and A), Bunda (SAT1, SAT2 and type A) and Same (SAT1, SAT2, type A and O) districts in Northern parts of Tanzania. The findings of this study reveal the existence of at least four serotypes (A, O, SAT1 and SAT 2) in the northern parts of Tanzania. The existence of multiple serotypes and multiple infections complicates control of FMD in the region. Further in-depth studies, including sequencing, are required to clarify the molecular epidemiology of FMDV in the region hence recommendation for appropriate control measures of FMD in Tanzania.
dc.description.sponsorshipThe Southern African Centre for Infectious Disease Surveillance (SACIDS) through the Wellcome Trust Grant WT087546MA.
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/20.500.14820/7485
dc.language.isoen
dc.publisherSokoine University of Agriculture
dc.subjectFoot disease virus
dc.subjectMouth disease virus
dc.subjectNorthern part-Tanzania
dc.titleTyping and characterization of foot-and-mouth disease virus detected in northern part of Tanzania
dc.typeThesis

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