Morphological and molecular genetic diversity of common bean (phaseolus vulgaris l.) varieties and landraces in Tanzania
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Date
2014
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Sokoine Uninversity of Agriculture
Abstract
Common bean (Phaseolus vulgaris L.) is among the most important crops in Tanzania.
The production level has been increasing from 626 340 tonnes to 780 000 tonnes from
year 2005 to 2012 respectively. Bean cultivation is highly adopted by small-scale and
large-scale farmers. More than one hundred common bean genotypes including varieties
and landraces have been collected from different geographical location in Tanzania, and
little information is known about their variations. This study characterized sixty-four
common bean genotypes by using morpho-agronomic traits and molecular genotyping by
using SSR marker. Twenty morpho-agronomic traits were evaluated by using the
common bean descriptor of IBPGR. Allelic loci were analyzed by using twenty-five SSR
molecular markers. Among the major findings, the Principal component analysis (PCA)
for the morpho-agronomic traits accounted 31% of the total variation of the genotypes
analyzed; while the PCA from molecular data accounted for 37% cumulative variations
for the common bean varieties and landrace analyzed. Results obtained enable the
identification of the duplicates in our collection, separating the Mesoamerican gene pool
from the Andean gene pool, identifying the relation between the varieties cultivation
with their grouped landraces. This information helped in determining the pedigree of the
background parent and were able to identify genotypes with important agronomic traits
which plant breeders can use them in breeding common bean variety with more desired
traits.
This work is expected to be published in Academic Journal of African Journal of Biotechnology (AJB).
Description
Dissertation
Keywords
Morphological, Molecular genetic diversity, Common bean, Landraces, Tanzania, Phaseolus vulgaris L.