Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia

Simple item page
dc.contributor.authorNyirenda, S. S.
dc.contributor.authorHang’ombe, B. M.
dc.contributor.authorKilonzo, B. S.
dc.contributor.authorKabeta, M. N.
dc.contributor.authorCornellius, M.
dc.contributor.authorSinkala, Y.
dc.date.accessioned2018-06-20T06:12:32Z
dc.date.available2018-06-20T06:12:32Z
dc.date.issued2016
dc.descriptionTropical Doctoren_US
dc.description.abstractPlague is a re-emerging zoonotic disease caused by the bacterium Yersinia pestis. The disease has caused periodic global devastation since the first outbreak in the 6th century.Twomonths after a suspected plague outbreak inNyimba district, sampleswere collected from94livestock(goats andpigs), 25 rodents, 6 shrews and33 fleas. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) techniques were used to investigate the presence of Y. pestis, which showed that 16.0% (4/25) of rodents, 16.7% (1/6) of shrews (Crocidura spp) and 6.0% (5/83) of goats were positive for IgG antibodies against Fraction 1 antigen of Y. pestis. Plasminogen activator (Pla) gene (DNA) of Y. pestis was detected in five pools containing 36.4% (12/33) fleas collected from pigs (n¼4), goats (n¼5) and rodents (n¼3). The detection of Pla gene in fleas and IgG antibodies against Fraction1 antigen in rodents, shrews and goats suggest that Y. pestis had been present in the study area in the recent past.en_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/2415
dc.language.isoenen_US
dc.publisherSageen_US
dc.subjectDomestic pigsen_US
dc.subjectFleasen_US
dc.subjectGoatsen_US
dc.subjectPlagueen_US
dc.subjectRodentsen_US
dc.subjectZambiaen_US
dc.titleMolecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambiaen_US
dc.typeArticleen_US
dc.urlhttp://tdo.sagepub.comen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Paper 2. Trop Doct-2016-Nyirenda-0049475516662804.pdf
Size:
291.32 KB
Format:
Adobe Portable Document Format
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.66 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Articles, Conference and Workshop Papers Collection