Identification and characterization of the interactive proteins with cytotoxic T-lymphocyte antigen-2α
| dc.contributor.author | Luziga, C. | |
| dc.contributor.author | Yamamoto, Y. | |
| dc.contributor.author | Yamamoto, M. | |
| dc.contributor.author | Nga, B. T. | |
| dc.contributor.author | Kusakabe, K. T. | |
| dc.date.accessioned | 2018-06-11T06:38:04Z | |
| dc.date.available | 2018-06-11T06:38:04Z | |
| dc.date.issued | 2014-12-17 | |
| dc.description.abstract | Cytotoxic T-lymphocyte antigen-2α (CTLA-2α) is a potent inhibitor of cathepsin L-like cysteine proteases. Recombinant CTLA-2α is known to be a potent, competitive inhibitor of cathepsin L-like cysteine proteases. In this study, cathepsin L, cathepsin C, and tubulointerstitial nephritis antigen-related protein 1 (TINAGL1) were identified as novel interactive proteins of CTLA-2α by the yeast two-hybrid screening system. The direct interactions and colocalization of these proteins with CTLA-2α were confirmed using co-immunoprecipitation and immunofluorescence staining, respectively. The disulfidebonded CTLA-2α/cathepsin L complex was isolated from mouse tissue. CTLA-2α was found to be specific and consistently expressed on the maternal side of the mouse placenta. Double immunofluorescence analysis showed that CTLA-2α was co-localized with cathepsin L, cathepsin C, and TINAGL1 in placenta. A simple cell-based fluorescence assay revealed that CTLA-2α exhibited inhibitory activity toward cathepsin C in live cells, which indicated that CTLA-2α is a novel endogenous inhibitor of cathepsin C. | en_US |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/2274 | |
| dc.language.iso | en | en_US |
| dc.subject | CTLA-2α | en_US |
| dc.subject | Cathepsin L | en_US |
| dc.subject | TINAGL1 | en_US |
| dc.subject | Proteases inhibitor | en_US |
| dc.subject | Cathepsin C | en_US |
| dc.title | Identification and characterization of the interactive proteins with cytotoxic T-lymphocyte antigen-2α | en_US |
| dc.type | Article | en_US |
| dc.url | http://dx.doi.org/10.1080/09168451.2014.991686 | en_US |