Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania

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dc.contributor.authorMaganira, Justine Daudi
dc.contributor.authorKidima, Winifrida
dc.contributor.authorMwita, Chacha John
dc.contributor.authorHalvarsson, Peter
dc.contributor.authorHöglund, Johan
dc.date.accessioned2021-08-06T05:20:20Z
dc.date.available2021-08-06T05:20:20Z
dc.date.issued2020
dc.descriptionJournal articleen_US
dc.description.abstractThe presence ofTaenia solium DNA from eggs in soils around the households in four Tanzanian villages in Kongwa district were analysed in relation to seasonal fluctuations and infection risk implications. A total of 192 pooled soil samples from five sampling points per household were examined by droplet digital Polymerase Chain Reaction (ddPCR) from 96 pigkeeping households both during the dry and rainy seasons. The pooled samples were first processed by a flotation-double sieving technique, followed by screening for worm DNA employing universal primers targeting the mitochondrial cytochrome c oxidase subunit I (cox1) gene of human taeniid species and some other helminths. All DNA positive samples were later confirmed by a specific ddPCR probe assay targeting the mitochondrial cox1 gene of T. solium. A total of 17.2% (n = 33) samples were positive with the universal ddPCR, whereas T. solium DNA was confirmed by the specific ddPCR only in 3.1% (n = 3) of the surveyed households. The detection of T. solium DNA in this study spells out a low risk of exposure to T. solium eggs from contaminated household soil. Based on our results, ddPCR seems to be a promising technology for screening T. solium eggs in soil.en_US
dc.identifier.citationJustine Daudi Maganira , Winifrida Kidima , Chacha John Mwita , Peter Halvarsson & Johan Höglund (2020) Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania, Infection Ecology & Epidemiology, 10:1, 1772668, DOI: 10.1080/20008686.2020.1772668en_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/3835
dc.language.isoenen_US
dc.publisheraylor & Francis Groupen_US
dc.subjectTaenia soliumen_US
dc.subjectEggsen_US
dc.subjectSoil contaminationen_US
dc.subjectddPCRen_US
dc.subjectKongwaen_US
dc.subjectTanzaniaen_US
dc.titleSoil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzaniaen_US
dc.typeArticleen_US
dc.urlhttps://doi.org/10.1080/20008686.2020.1772668en_US

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