Evaluation of microbial contamination along the milk value chain in two districts of Tanzania
| dc.contributor.author | Hyera, Emil | |
| dc.date.accessioned | 2017-12-08T10:40:12Z | |
| dc.date.available | 2017-12-08T10:40:12Z | |
| dc.date.issued | 2015 | |
| dc.description | MSc Thesis | en_US |
| dc.description.abstract | Milk is important as a valuable diet, but due to its nutritional value and perishable product it serves as an ideal medium for growth of various microorganisms under suitable conditions, hence it is a staple food in epidemiology linked to zoonotic pathogens. This study was carried out in two districts in Tanga region (Northern Tanzania) to estimate microbial load, isolate selected pathogens and establish their possible sources or entry along the milk value chain. A total of 114 respondents were interviewed and subsequently milk samples were aseptically collected for laboratory microbial analyses using the standard ISO procedures for Food microbial analyses — Horizontal methods. The results revealed poor practices and lack of formal training on milk hygiene among most of the actors. More than 90% of all handled milk samples had Total plate count (TPC) above the EAC maximum acceptable standard of 2.0x10 5 CFU/ml. The overall mean coliform plate count (CPC) was 1.8x10 6 ± 6.2x10 6 CFU/ml, which indicated poor animal husbandry and hygiene practices. The values of TPC and CPC between independent variables were not statistically significant different (P > 0.05). In the samples, one contained CPS isolate counting to 5.1x10 5 CFU/ml likely to cause staphylococcal poisoning. Isolated bacteria were Escherichia coli, Staphylococcus aureus and Listeria spp. including Listeria innocua, Listeria ivanovii and Listeria monocytogenes. Other microorganisms included Klebsiella spp., Proteus spp., Staphylococcus spp., Enterococcus faecalis, Bacillus cereus and Pseudomonas spp. In the identified pathogens, L. monocytogenes was most (42.1%) predominant. The quality of milk was poor; unhygienic practices, poor animal husbandry practices, organization of milk supply chains, dysfunction of the regulatory agencies and quality control structures predispose the public to risk of contracting milk-borne infections. Training on animal husbandry practices and public education on general milk handling and hygiene are recommended. Also, sector policies, organizational structures and support services and research into public health risks in milk must be focused. | en_US |
| dc.description.sponsorship | Ministry of Livestock and Fisheries Development, German Department for International Cooperation (GIZ) and CGIAR CRP4 Agriculture for Nutrition and Health through the Safe Food Fair Food II (SFFF II) project | en_US |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/1829 | |
| dc.language.iso | en | en_US |
| dc.publisher | Sokoine University of Agriculture | en_US |
| dc.subject | milk quality | en_US |
| dc.subject | hygienic practices | en_US |
| dc.subject | animal husbandry | en_US |
| dc.subject | perishable products | en_US |
| dc.subject | nutritional value | en_US |
| dc.subject | microbial contamination | en_US |
| dc.subject | milk value chain | en_US |
| dc.title | Evaluation of microbial contamination along the milk value chain in two districts of Tanzania | en_US |
| dc.type | Thesis | en_US |