Abstract:
The present study was conducted to investigate the presence of Arenaviruses from rodents,
shrews and elephant shrews captured in selected wildlife-human interfaces in Tanzania.
The study involved six sites with high potential for contact between wildlife and humans
namely; Ruaha, Kilombero, Mtwara, Mbeya, Mbinga and Mikumi. A total of 121 animals
comprising 111 rodents, 3 shrews and 7 elephant shrews were screened for Arenaviruses
using conventional Polymerase Chain Reaction (PCR). The genetic relatedness of
Arenaviruses was evaluated by conducting phylogenetic analysis of partial sequences of
the S gene. The association between age and sex with the presence of Arenaviruses was
assessed. Of the 121 animals, 7 (5.8 %) were shedding Arenavirus. All positive animal
samples were obtained from the Ruaha site at crop raiding and peridomestic interfaces.
Eighty six percent of the infected animals were Mastomys sp. and 14 % were Arvicanthis
sp. Age and sex of the animals were not significantly associated with occurrence of
Arenaviruses in rodents, shrews and elephant shrews (P > 0.05). Additionally, Arenavirus
detection in fecal specimens was not significantly different from the detection in
oropharyngeal specimens (P > 0.05), clearly indicating that both specimens are useful for
Arenavirus testing. Phylogenetic analysis showed that isolates obtained from this study
were related to the Old World Arenaviruses, and include strains of Morogoro virus and a
novel strain of Arenavirus. In conclusion, the present study has confirmed the presence of
Arenaviruses closely related to other known Old World Arenaviruses in the Ruaha
ecosystem.
