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Item Acceptability of soup powders made from selected traditional leafy vegetables grown in Lindi, Tanzania(2021) Abdallah, A.; Chove, L. M.A study was conducted to assess the acceptability of soup formulated from traditional leafy vegetables (TLVs) grown in Lindi, Tanzania. Three TLVs, Amaranth hybrids known as amaranthus leaves (AML), Manihortesculenta known as cassava leaves (CAL) and Ipomeabatatas known as sweet potatoes leaves (SPL) which had been optimized for Iron content, were used to prepare 4 vegetable soup formulations (F1– 60.0:7.5:22.5); (F2 –70.0:5.0:15.0); (F3 –80.0:2.5:7.5) and (F4 –40.0:10.0:40.0) respectively. Descriptive sensory analysis was performed by10 trained panelists who used 5 descriptors to quantitatively describe the sensory characteristics of four soup formulations. Thirty consumers assessed the degree of liking of products’ sensory attributes using a 7-point hedonic scale. External preference mapping was performed by relating sensory data with hedonic responses. Mean intensity ratings of descriptive attributes of the soup showed that F1, F2 and F3had significantly higher(p<0.05) mean intensity scores in colour, aroma, and mouth feel than F4. The consumer study showed that, with exception of mouth feel, consumers showed significant differences (p<0.05) between samples in colour, aroma, taste and overall acceptability. It was thus concluded thatF1 was the most liked by consumers due to colour, aroma and mouth feel followed by F2 and then F3 and finally F4. Furthermore, the preference mapping results showed that colour, aroma and mouth feel attributes were the main drivers for positive consumer preference for vegetable soup. Thus, selection and processing of vegetables, which retain these attributes, is of greater importance for consumer acceptability and hence increased utilization for consumer’s health and well-being.Item Application of slightly acidic electrolyzed water as a potential sanitizer in the food industry(Hindawi, 2024-02-29) Issa-Zacharia,AbdulsudiTe food industry has extensively explored postharvest microbial control, seeking viable technologies to ensure food safety. Although numerous chlorine-based commercial sanitizers serve this purpose, many are plagued by constraints such as instability and diminished disinfectant efcacy. Tese issues arise from exposure to organic matter in wash water, light, or air. As an innovative and promising alternative, slightly acidic electrolyzed water (SAEW) has emerged, captivating attention for its robust sterilization potential and eco-friendliness in agricultural and food sectors. SAEW generated via electrolysis of a diluted hydrochloric acid (HCl) solution with concentrations ranging from 2 to 6% or aqueous solution of sodium chloride (NaCl) in a nonmembrane electrolytic chamber is reported to possess equivalent antimicrobial properties as strong acidic electrolyzed water (StAEW). In contrast to traditional chlorine sanitizers, SAEW leaves less chlorine residue on sanitized foods such fresh-cut fruit and vegetables, meat, poultry, and aquatic products due to its low available chlorine concentration (ACC). Its near neutral pH of 5 to 6.5 not only renders it environmentally benign but also mitigates the production of chlorine gas, a contrast to low pH conditions seen in StAEW generation. Te bactericidal efect of SAEW against various strains of foodborne pathogens is widely believed and accepted to be due to the combined action of high oxidation-reduction-potential (ORP) reactions and undissociated hypochlorite/ hypochlorous acid (HOCl). Consequently, a burgeoning interest surrounds the potential of SAEW for sanitation in the food industry, ofering an alternative to address shortcomings in sodium hypochlorite solutions and even StAEW. It has been hy- pothesized from a number of studies that SAEW treatment can increase the quality and nutritional value of harvested fruits, which in turn may enhance their ability to be stored. Terefore, SAEW is not only a promising sanitizer in the food industry but also has the potential to be an efcient strategy for encouraging the accumulation of bioactive chemicals in plants, especially if it is used extensively. Tis review encapsulates the latest insights concerning SAEW, encompassing its antimicrobial efectiveness, san- itization mechanism, advantages vis-à-vis other sanitizers, and plausible applications across the food industryItem Assessment of bacterial contamination and associated risk factors in pork slaughtered and marketed in urban Tanzania(Tanzania Journal of Health Research, 2023-10) Nonga Cosmas; Zacharia Issa; Mkupasi Ernatus; Ngowi HelenaIntroduction: In Tanzania, pork consumption is increasing and become popular in urban areas creating a good market for pigs raised in rural areas. However, little is known regarding the microbial safety of the marketed pork in the country. This study assessed the level of bacterial contamination and contributing factors in pork slaughtered and sold in Arusha, Dar es Salaam and Dodoma Tanzania through Total Viable count, Coliform count, Escherichia coli and Staphylococcus aureus count. Methods: A cross-section study was conducted and a total of 90 pork samples were collected from pig slaughter facilities, pork centres and butcheries. Standard methods for microbial analysis in food products (ISO 7218:2007(E)) were used. Results: It was revealed that all (100%) pork samples had bacteria contamination with an overall mean for total viable count of 5.93±1.50 log CFU/g, and coliform forming unit of 4.30±1.14 log CFU/g. Over 92.2% of the pork samples were contaminated by E.coli with a mean count of 3.12±1.33 and S. aureus was isolated in more than 84.4% with a mean count of 2.71±1.34 log CFU/g. The mean values were higher than the limit set by Tanzania Standard (TBS/AFDC 22 (5266) P3). In addition, 79.4% of the pork slaughter facilities and selling points surveyed had poor hygiene and lacked safety and quality control measures. Conclusion: Thus, it may be deduced that the pork carcasses assessed were of poor microbiological quality posing a health risk to pork consumers. To minimize public health risks, food control authorities should ensure effective enforcement of policies and regulations in controlling pig slaughtering and pork marketing in the country. Also, education on hygienic practices for all stakeholders along the pork value chain should be provided.Item Assessment of compliance with good agricultural practices in pig farming in Mpwapwa and Mbulu districts, Tanzania(Taylor & Francis Group, 2023-07-14) Nonga, Cosmas; Zacharia, Issa; Mkupasi, Ernatus; Ngowi, HelenaA cross-sectional study was conducted between February and May 2022 to explore the pig farming practices and assess their compliance with Good Agricultural Practices (GAPs) and their associated constraints in Mpwapwa and Mbulu districts. A total of 136 pig farmers were randomly chosen from purposively selected wards for interview using a structured questionnaire. The study revealed that 71.3% of all pig farmers were aware of GAPs on pig farming, but only 19.8% of the pig farmers complied with the stipulated GAPs. About 81.6% of the respondents had poor piggery hygiene and 76.5% had poor piggery conditions. Pig farmers reported the cost of feeds (83.8%) and veterinary services (81.6%) to be the hindrance to compliance with GAPs. It was concluded that pig farmers are aware of GAPs but not all implement them. Limited availability and high costs of pig feeds and veterinary services are among the factors limiting the full implemantation of GAP in pig farming in the Mpwapwa and Mbulu districts. Therefore, there is a need for the Ministry of Livestock and Fisheries Development and private investors to collaborate to ensure accessibility to Agro-vet services in rural areas with minimal cost. A positive effect could also result from encouraging pig farmers to use locally available ingredients to compose pig feeds.Item Assessment of knowledge, attitudes and practices on breastfeeding among HIV infected mothers with infants in Kibaha town council(Sokoine University of Agriculture, 2011) RWERAMIRA,MARGARETH CLEMENTKnowledge, Attitudes and Practices (KAP) on breastfeeding among HIV-infected mothers are important in determining the health and nutritional status of their children. The HIV pandemic and the risk of mother-to-child transmission (MTCT) of HIV, especially through breastfeeding (BF) poses special difficulties to HIV-infected mothers, their infants and the community. This study was designed to assess KAP on breastfeeding among HIV-infected mothers having children aged 0-12 months living in Kibaha Town Council. A cross-sectional, descriptive study design was employed and convenient sampling procedure was used to select the respondents. Primary data were collected using structured questionnaire which were administered by face to face interview. Data analysis was done using Statistical Package for the Social Science (SPSS) 15.0 version computer software. The study findings revealed that there is limitation in terms of KAP on BF, complementation and infant feeding options among the HIV-infected mothers. Breastfeeding was chosen by majority of respondents (96%) compared to replacement feeding (6%). About 76% of mothers ceased to practice EBF before their infants were six months old due to fear of MTCT of HIV. The study also revealed that mothers’ level of education was significantly associated with their knowledge (p = 0.00403). Mixed feeding (63.6%) and nipple cracks (77.8%) were the most known factors by the respondents to increase the risk of MTCT of HIV. Breast conditions such as sore/cracked nipples; fear of stigma; lack of counselling and follow up from the health workers; and cultural beliefs such as BF by using one breast were observed as the constraints faced by the respondents. Counselling services on infant feeding options, regular follow to mothers and more training to the counsellors on BF and PMTCT of HIV need to be strengthenedItem Bushmeat consumption in Africa: a microbiological safety challenge?(Asian Food Science Journal, 2023-09-12) Ahouanse Gwladys Gloria Amen; Issa-Zacharia Abdulsudi; Majaliwa NuriaObjective: This review analyzed the microbiological safety of bushmeat consumed in Africa over the past decades. Methodology: Previous Studies mainly focused on large animals like antelopes and smaller ones like grasscutter. Most microbes studied were similar to those in domestic animal meat, except for rare pathogens such as Salmonella spp, E.coli. Sampling, experiments, and microbe prevalence varied among studies. Results: All studies confirmed the presence of zoonotic pathogens dangerous to human beings. Therefore, more investigations are needed, especially for the chronic and severe cases of pathogens, since only few studies have addressed the bushmeat's microbiological safety in Africa. Conclusion: Efforts should be made to improve bushmeat safety and public health in Africa. New policies and public regulations must be developed and implemented to ensure hygienic and legal bushmeat production in Africa.Item Chemical quality of stream and borehole water used by selected communities in Lushoto district, Tanzania(An International Journal of Basic and Applied Research, 2020) Hadija, A.; Chove, L.The chemical quality of water in two streams and three boreholes from Sunga and Mbaru wards in Lushoto, Tanzania was investigated. Water was collected at three locations along the stream, including forest areas, populated area with agricultural activities and less populated areas with agricultural activities. Borehole’s water was collected from three boreholes found in each ward and analyzed for pH, hardness, BOD, phosphates, nitrates by standard methods. Nested design was applied. Data obtained was analyzed by R-Software for ANOVA. Means were separated by Tukey‘s honest at p<0.05. Significant differences (p<0.05) in all chemical parameters (pH: 6.92- 7.34; Hardness; 23.88+64.43;Nitrite 1.73 -19.80; Phosphate 0.58-3.90; Ammonia 0.02-0.08; Lead -0.001-0.002) were observed in locations within the streams except arsenic. DDT was not detected. With the exception of phosphate in both streams, all water samples tested met TZS (2016) standards and WHO (2011) guidelines. This implies that stream water is considered unsafe for use since it exceeded the maximum phosphate limit by TZS (2016). All parameters tested in borehole water met the requirements for both standards except pH and ammonia which exceeded the WHO water guideline. It is recommended that the communities be educated on the best practices to keep water sources safe.Item Colour and antioxidant activities of honey from different floral sources and geographical origins in Tanzania(Tanzania Journal of Agricultural Sciences, 2016) Shekilango, S. G; Mongi, R. J; Shayo, N. BHoney is a sweet, yellowish liquid that is produced by honey bees and it has been used for many years for nutrition and therapeutic purposes. Its therapeutic potential has been associated to its antioxidant capacity which plays an important protective role in human health through scavenging of free radicals in the human body and thereby decreases the amount of free radical and damage to biological molecules like lipids and DNA. This study was carried out to investigate the effect of floral source (miombo and acacia) and geographical origin (Central, Coastal, Lake, Northern, and Southern Highland) in mainland Tanzania on colour and antioxidant activity of honey samples. Colour, total flavonoids content (TFC), total phenol phenolic contents (TPC) and antioxidant activities of the samples were evaluated using UV/Vis spectrophotometer, Folin-Ciocalteu reagent and Ferric Reducing Antioxidant Power (FRAP) methods respectively. There were significant (p<0.05) differences in all parameters assessed between floral sources. Miombo honey samples had higher pfund values (mm) for colour ranged from 31.55 mm (white) to 143.98 (dark amber) than acacia samples with values ranged from 17.33 (extra white) to 62.28 mm (light amber). Miombo samples had higher TFC values of 13.5±0.25-39.9±0.42 mgRE/100g DM) and TPC of 127.9±2.5-395.2 mg GAE/100g DM) than acacia samples with values of 12.7±0.60-17.5±0.38 mgRE/100g DM and 119.5-168.2 mg GAE/100g DM respectively. Similarly, significantly (p<0.05) highest and lowest FRAP values of 488.9-956.3 and 252.6-368.26 μM Fe2+/100g DM were observed in Miombo and acacia honey samples respectively. Moreover, variations in colour, TFC, TPC and FRAP between zones were significant (p<0.05). Within the miombo samples, northern and coastal zones had respective lowest and highest values whereas central and northern zones had respective lowest and highest values within the acacia samples. A strong correlation (R2= 0.942) between TPC and antioxidant activities of honey samples suggest that the antioxidant of honey is highly linked to TPC. Therefore, the study has revealed that, floral sources and geographical origins have varied significant effects on colour, flavonoids, total phenols and antioxidant activities of Mainland Tanzania honey. Honey samples from miombo floral source had higher antioxidant activities and hence their consumption is more recommended.Item Compliance by SMEs to existing Tanzania standards with respect escherichia coli and staphylococcus aureus in cultured milk: a case of Dar es salaam, Tanzania(An International Journal of Basic and Applied Research, 2021) Samwel, J.; Chove, L. M.Small and medium enterprises are important for economic growth not only in Tanzania but all over the world. Most of them are informal, without official registration. However, they provide tremendous contribution to food security, nutrition, income generation and poverty reduction in the country. Therefore, upgrading and modernization of Small and Medium Enterprises (SMEs) to ensure food security, safety and quality of food has becomes one of the priorities of Tanzania development visions of 2025, which is the blueprint of structural economic transformation of the country. To help achieve this, the present study was conducted to assess the safety of cultured milk. A total of 33 cultured milk samples were collected from 11 SMEs which were analyzed in triplicate to make 99. Two microorganisms, Escherichia coli and Staphylococcus aureus were analysed in cultured milk. Results were compared against the existing Tanzania standards. The isolates had an overall mean of 2.02 × 102 ± 1.73 × 102CFU/g, 1.91×102 ± 1.85 ×102CFU/g respectively for E. coli and S. aureusc count. The results obtained were higher than the limits set by the Tanzanian Standard (TZS1625:2013). It may thus be concluded that sampled cultured milk was of poor microbial quality and hence not safe for consumption. Poor hygienic practices during milk handling pose a high risk to cultured milk and render it unsafe for human consumption.Item Detection of proteolysis in high temperatures treated milk by reverse phase high performance liquid chromatography (RP-HPLC)(Academic Journals, 2014-02) Chove Lucy M.; Grandison S. Alistair; Lewis Michael J.; Issa- Zacharia AbdulsudiProteolysis of UHT milk during storage is one of the problems affecting the dairy industry worldwide. Native enzymes have been implicated as being the main cause of spoilage of such milk. In the current study, reverse phase high pressure chromatography (RP-HPLC) method was used to detect proteolysis by native enzymes in high temperature heated milk. The aim of this research was to assess susceptibility of milk to proteolysis by native enzymes, after being subjected to various temperature- time processing conditions. Samples of raw and heated milk were clarified prior to analysis. Clarification was carried out to obtain pH 4.6 and 6% TCA soluble extracts, which were injected into RP- HPLC after filtration. This method confirmed that raw milk and milk processed at 85°C /15 s were the most proteolysed, indicating that the high temperatures employed during this study (110, 120, 130 and 142°C for 2s) inactivated the native enzymes. The RP-HPLC method is a useful method for the detection of proteolysis in milk.Item Detection of proteolysis in milk by pseudomonas fluorescens using urea PAGE method(Macrothink Institute, 2017-12-28) Chove, Lucy Mlipano; Alistair, Grandison; Michael, LewisProteolysis of milk during storage by two strains of Pseudomonas NCIMB 702085 (414) and NCIMB 701274 (416) was investigated using the Urea PAGE method. Pseudomonas fluorescens enzymes were also extracted and purified by dialysis before inoculation into UHT skim milk in an attempt to partially purify the enzyme. Results showed that dialysis removed some peptides and amino acids which would interfere with the assay procedure. The method also confirmed that Pseudomonas fluorescens NCIMB 701274 (416) was more proteolytic than Pseudomonas NCIMB 702085 (414). Thus, Urea PAGE is a useful method for monitoring proteolysis in milk by Pseudomonas fluorescens.Item Development and sensory evaluation of yoghurt flavoured with solar dried fruits(Pearl Research Journals, 2017) Ndabikunze, B. K.; Mumba, F. G.; Ngowi, H.; Chove, L; Mongi, R; Abdulsud, I.A study of developing solar dried fruit (5%) flavoured yoghurts using three types of fruits was conducted to determine acceptability and shelf life of developed products. A total of six samples namely banana, mango, pineapple, banana/mango, banana/pineapple and mango/pineapple were used as flavours in yoghurts. They were added either as fruit pieces or as powders. Shelf life projection study suggested that developed yoghurt products could be stored for up to 21 days at 4 °C without undergoing spoilage. Plain yoghourt (control) was the most liked sample and scored highest mean values for all attributes, which was significantly different from all other samples (p<0.05). Among the fruit flavoured yoghourts, mango was the most preferred sample in terms of flavor, aroma and general acceptability whereas the pineapple was the least acceptable. Although yoghurts flavoured with fruits pieces scored higher mean values in all sensory attributes than yoghurts flavoured with powdered fruits, no significant differences (p>0.05) between the two forms of fruit flavours was found. This needs to be further investigated. Thus, although the control was the most liked sample, solar dried fruit can be successfully used as yoghurt flavour, which may improve the texture and nutritional quality of the formulated yoghurts, making them available throughout the year, hence increasing farmer’s income.Item Development of natural liquid and powdered meat tenderizer based on papaya peel, ginger and garlic(Science Publishing Group, 2024-04-12) Issa-Zacharia Abdulsudi; Muhimbula Happiness Samuel; Bikuba Anastazia Niyonkulu; Mato Joachim DottoRecently, the application of exogenous enzymes for meat tenderization has turned the attention of food technologists and meat scientists. This research investigates the synergistic impact of natural tenderizers derived from the combination of papaya peels (P), ginger (G), and garlic (Ga) on the sensory attributes of goat meat such as color, aroma, taste, tenderness and overall acceptability. The tenderizers were formulated in different concentration ratios of 3:2:1 in both liquid and powdered form. The sensory quality parameters for all tenderized goat meat samples under observation including the control, were evaluated by 60 semi-trained panelists using a five-point hedonic scale. The effect of tenderizers was compared to the control which was devoid of tenderizing ingredients. Tenderness was greatly improved (p<0.05) by tenderizer P:G:Ga (3:2:1) in both liquid and powder forms at 45- and 90- minutes of tenderization. Alternatively, liquid and powdered Ga:G:P (3:2:1) were highly significantly rated (p<0.05) in taste compared to all other tenderizer formulations for 45- and 90-minute tenderization. From the samples tested, the control sample in both setups was the least rated in all sensory attributes, except for color in powdered tenderization. Generally, liquid and powdered P:G:Ga (3:2:1) and Ga:P:G (3:2:1) tenderizers received significantly higher (p<0.05) overall acceptability scores than G:G:aP (3:2:1) and the control sample. While liquid tenderizers mostly performed better compared to powdered tenderizers for all sample combinations, there was a significant improvement (p<0.05) in tenderness when both liquid and powdered tenderizers were used for 90 minutes compared to 45-minute marination. This suggests that, the longer the tenderization time, the greater the effect on tenderness. These findings underscore the importance of natural tenderizers in enhancing consumer satisfaction with meat products,Item Diversity Of Underutilised Vegetables In Africa And Their Potential In The Reduction Of Micronutrient Deficiency:A Review(Science publishing Group, 2023) Zacharia,Abdulsudi Issa; Majaliwa,Nuria Kudra; Nyamete,Frida Albinusi; Chove,Lucy MlipanoMicronutrient deficiency affects all groups worldwide and is a major public health issue. It has devastating effects on all ages, especially in sub-Saharan Africa. It causes anemia, night blindness, increased mortality in children and pregnant women, osteoporosis in adults and rickets in children, lower infectious disease resistance, weariness, and cognitive impairment. Recent attention has focused on micronutrient deficits. Fortunately, these micronutrients are abundant in vegetables. Many African vegetables are endemic due to various weather variations. Diverse geographical areas on the continent allow for exotic and indigenous vegetable kinds. In Africa, many African Indigenous Vegetables (AIVs) are grown and eaten. Exotic veggies are popular in cities, but rural Africans still prefer indigenous crops. Despite their availability, most of the indigenous vegetables are underutilised and have not been adequately explored due to the decreased attention to their production, consumption and utilization. Due to their diversity, they have a great potential to contribute to food security by providing nutrient rich healthy diets. They are rich in vitamins and minerals and hence make a potential source of micronutrients that can help reduce the reported deficiencies. This paper provides a literature review on the wide variety of underappreciated vegetables grown in Africa, their health benefits, and their potential to alleviate micronutrient shortages. It also investigates the barriers to increasing consumption and explores some of the strategies that have been employed to address these problems. Due to their significant role in ensuring food and nutrition security, along with their rich nutrient composition, there exists an urgent imperative to enhance public awareness regarding their consumption and the attainment of nutritional security.Item Effect of depth and distance of the borehole from the septic tank on the physico-chemical quality of water(Macrothink Institute, 2017-12-28) Chove, Lucy Mlipano; Mongi, Richard; Chenge, LawrenceA study to examine the effect of depth and distance of the boreholes from the septic tank on physico-chemical parameters of the borehole water consumed in Dar Es Salaam, Tanzania was conducted. Samples were collected from 48 boreholes in Ilala, Kinondoni and Temeke districts and subjected to pH, B.O.D (Biological Oxygen Demand), total hardness, copper and lead analyses in relation to safety and quality levels set by WHO and TBS. Analysis by R-statistics indicated that pH and B.O.D significantly (p<0.05) decreased as the depth of borehole increased, whereas total hardness was significantly (p<0.05) higher in very deep boreholes than the other heights. Total hardness and pH were not significantly (p>0.05) affected by distance between septic tank and borehole and the B.O.D levels were significantly higher in the borehole water situated near the septic tank than those which were far. Based on the physico-chemical characteristics of water, 25% of the samples did not comply with WHO and TBS specification limits while 75% complied, most of which had shallow depths and were situated near the septic tank. It may thus be concluded that both the distance and the depth between septic tank and borehole influenced physico-chemical characteristics of water.Item Effect of processing methods on the micronutrient profile, colour, and anti- nutritive components of justicia heterocarpa (mwidu)(European Journal of Nutrition & Food Safety, 2025-02-05) Swai, Zenorina Aloyce; Nyamete, Frida Albinus; Silayo, Valerian. C. K.Justicia heterocarpa (mwidu) is a popular indigenous leafy vegetable picked wild in rural regions of Morogoro, Tanzania, during the wet seasons. This research examined the impact of processing on micronutrients, total phenols, and anti-nutrient content. The fresh leaves (FL) underwent direct shade drying (UBLDDR), blanched shade drying (BLDR), blanched oven drying (BLDO), fermentation (FFL), and gas and microwave cooking (FLCO5, FLCO10, and FLMCO2). Vitamins, chlorophyll, minerals, phenolic compounds, and anti-nutrients were analyzed. All laboratory experiments adhered to procedures and guidelines. The pH dropped more significantly to <3.5 in a 3% salt-3% sugar brine solution with 1.328 ± 0.006 mg/100 g of lactic acid compared to its counterpart. Blanched leaves dried in 5 days, but unblanched leaves took 15 days. Fermented samples demonstrated a notable reduction in total chlorophyll concentration (0.0964 ± 0.075 mg/g) compared to other processing techniques. The nutritional and anti-nutritional composition of Justicia heterocarpa showed significant change (P < 0.05) depending on processing methods. The results indicated a significant loss of vitamin C in the fermented and ten-minute cooked samples, at 74.57% and 61.64%, respectively.Cooked FLMCO2 (107.4%, 11.29 ± 0.03 mg/100g) and FLCO5 (86.26%, 10.51 ± 0.02 mg/100g) exhibited more than a two-fold increase in beta-carotene compared to fresh leaves (3.67 mg/100g). In comparison to alternative processing procedures, samples cooked for 10 minutes exhibited significant mineral leaching, whereas unblanched direct shade drying preserved the highest mineral concentration. Fermented samples (532.83 ± 14.91 GAE/100 g) exhibited a 64.19% increase in total Phenolic compounds compared to fresh leaves (190.83 ± 14.91 GAE/100 g). Nonetheless, tannins increased by 68.1% (254.44 ± 7.45 GAE/100g) in the fermented samples. Samples exposed to extended cooking (17.8 ± 3.17 mg/100 g) and fermentation (40.28 ± 3.34 mg/100 g) exhibited the lowest levels of phytates. The oxalate concentration was significantly decreased in the cooked samples. Justicia heterocarpa may serve as a sustainable food supply in areas of Tanzania experiencing nutritional deficits.Item Effect of solar drying methods on total phenolic contents and antioxidant activity of commonly consumed fruits and vegetable (mango, banana, pineapple and tomato) in Tanzania(Academic Journals, 2015-05) Mongi, Richard J.; Ndabikunze, Bernadette K.; Wicklund, Trude; Chove, Lucy M.; Chove, Bernard E.The effects of solar drying methods [Cabinet direct (CDD), cabinet mixed mode (CMD) and tunnel (TD) drying] on total phenolic content (TPC) and antioxidant capacities of commonly consumed fruits and vegetable in Tanzania were investigated. The TPC and FRAP in mangoes (Mangifera indica cv. Dodo, Viringe and Kent), bananas (Musa acuminata, cv. Kisukari, Kimalindi and Mtwike), pineapples (Ananas comosuss cv Smooth cayenne) and tomatoes (Lycopersicum esculentum cv. Tanya, Cal J and Onyx) were evaluated using Folin-Ciocalteu reagent and ferric reducing antioxidant power (FRAP) methods, respectively. There were significant (p<0.05) variations in TPC (mg GAE/100 g DM) and FRAP (μmol/100 g DM) among the fresh fruit and vegetable samples. The highest TPC was in tomato (476.6±8.6 - 538.9± 1.4) and lowest in banana (139.3±2.3 - 189.2±2.7). Drying methods had significant (p<0.05) effect on TPC and antioxidant values of the samples. All fresh samples had higher TPC levels but declined significantly in dried samples with the exception of tunnel dried tomatoes. Among the dried samples, the tunnel dried samples had less TPC loss (6-16%) than the cabinet dried samples (17-42%). The cabinet direct and mixed mode samples were not statistically different (p>0.05) from each other. However, tunnel dried samples had less FRAP loss (6-13%) which were statistically different (p<0.05) from the cabinet dried samples (14-56%). Percentage TPC and FRAP recoveries (%) differed significantly (P<0.05) between the varieties within the fruits/vegetable for both drying methods. A strong 2 2 correlations between TPC and FRAP in both fresh (R = 0.970) and dried samples (R =0.8636) suggests that solar drying methods have significant effects on total phenolic contents and antioxidant activities of fruits and vegetables with tunnel drying method giving significantly less effects.Item Effects of dietary supplements of DHA-enriched micro algae diet on physical and technological properties of dairy cow milk fat(2023-08-25) Issa-Zacharia AbdulsudiThis study examined the effect of dietary supplementation of DHA-enriched micro algae diet on physical and technological properties of dairy milk fat in terms of the dynamic crystallization and melting behaviour. Two dairy cows were subjected to feeding regime of DHA-enriched micro algae diet and control diet. The experiment was carried out during 21-d to determine the normal (control) and DHA-enriched micro algae diet modified milk fat that were taken for further analysis. The melting and crystallization behaviour of the milk fat from the cows fed control and DHA-enriched micro algae diets was studied using differential scanning calorimetry (DSC), Q1000 (TA Instruments, New Castle, DE, United States). DHA-enriched micro algae supplementation strongly affected the melting and crystallization properties of milk fat. Generally, the onset temperature (°C) of milk fat crystallization was significantly lower in DHA-enriched milk fat as compared to the control. The quantity of heat released by fat crystallization expressed as J/g (peak area) was significantly lower in enriched milk fat. DHA-enriched milk fat also had a lower peak maximum temperature as compared to control in all samples investigated. All melting curves displayed two peaks (lower melting and higher melting peaks) and for melting peaks, DHA-enriched milk fat melted at significantly lower temperature as compared to the control indicating an increase in the degree of unsaturation of milk fat. Melting offset temperature was significantly lower for DHA- enriched milk fat as compared to the control. It can be concluded that from the results of this study, micro algae supplementation significantly altered the milk fat composition and positively affected melting and crystallization behaviour of milk fat.Item Epidemiology of tuberculosis in inmates of selected central prisons in Tanzania(Sokoine University of Agriculture, 2014) RUGAMBA,WILSON EMMANUELA cross sectional study was carried out to determine prevalence of tuberculosis (TB) among prison inmates and assessed risk factors contributing to the disease in five selected Central prisons namely; Butimba, Isanga, Ruanda, Ukonga and Segerea in Tanzania. The prevalence was established based on sputum smear microscopic examination for Acid Fast Bacilli (AFB). Questionnaires were used to gather information from prison inmates and prison staff about possible risk factors for TB spread in prisons. Due to the current association between HIV infection and TB epidemic, HIV screening was carried out by testing capillary blood adhering to the National algorithm whereby two serial rapid antibody tests namely Alere Determine and Uni-Gold were used. A total of 370 prisoners out of 8330 inmates were involved and the overall prevalence of TB was 3.8%. On commencement of the survey, 71 prisoners (0.85%, n=71; N=8330) from studied prisons were under anti TB treatment. The overall prevalence of HIV in studied Prisons was 5.4%. Il was also noted that overcrowding, poor ventilation, poor prison architecture, history of TB contact, HIV infection, smoking cigarettes, and limited knowledge on TB to prisoners were the risk factors for TB in prisons. The present study confirms the high prevalence of pulmonary tuberculosis in prison populations, thus suggest not only active transmission of the disease in the prison settings but also the need for executing urgent preventive measures.Item Erosion hazard assessment in the upper Ewaso Ng’iro basin of Kenya: application of gis, usle and eurosem(Sokoine University of Agriculture, 1999) Mati Bancy MburaA methodology was developed for assessing soil erosion hazard in the Upper Ewaso Ng’iro basin of Kenya, using Geographic Information Systems (GIS), the Universal Soil Loss Equation (USLE) and the European Soil Erosion Model (EUROSEM). The USLE was used in a GIS environment by creating thematic maps of R, K, L, S, C and P and then calculating soil loss by raster-grid modelling with Arc/Info GRID. The rainfall erosivity factor (R) was derived from relationships between rainfall amount and erosivity using erosion plot data from within the catchment. The nature of the relationship was found to be a function of agi o-climatic zones of the region. Mean annual erosivities ranged from 145 to 990 J m'2 hr'1. For a given amount of rainfall, erosivity was higher in zone IV than in the wetter zones 11-111. The soil erodibility factor (K) was estimated using the USLE nomograph and data from laboratory analysis of field samples collected from representative major soil mapping units. The K-values were low to medium, ranging from 0.10 to 0.25 over 84 percent of the basin. The topographic factor (LS) was obtained by creating Digital Elevation Models (DEMs) of the basin with TOPOGRIDTOOL of Arc/Info. These were then used to determine the slope steepness and length factor values, calculated with raster-grid modelling. Although DEMs proved a useful tool, maximum values of both steepness and length had to be set in this reconnaissance study to achieve reasonable results. A finer resolution of input data and a smaller grid cell size are needed for accurate determination. The cover and management factors (C) were obtained by determining the land cover types within the basin using remotely sensed data (SPOT 1 colour composite prints) and ground truthing studies. The factor values were estimated from USLE guide tables and measurements of cover from plots and test sites. Some 70 percent of the basin is covered by rangelands. The conservation practice (P) factor values were estimated from USLE guide tables and then applied to areas where soil conservation had been introduced /?. M. Mali PM > Thesis 1999 Silsoe College Cranfield .■Ihstract •'university II according to maps obtained from the Ministry of Agriculture. The USLE was validated using data from erosion plots. A value of R2 = 0.645 was obtained between predicted and measured values but the standard error was rather high (e = 5.745 t ha'1 yr'1). Using an annual soil loss of 9.0 t ha'1 yr'1 as tolerance level, some 36 percent of the basin was found to experience unacceptably high erosion rates. Most of this area was communal grazing land and cropland where soil conservation measures had not been applied. A critical land cover type within the grazing land is shrubland, where vegetation cover is less than 40 percent and high erosion risk was predicted and confirmed by field surveys. EUROSEM could not be integrated within a GIS in the time available for research. It was therefore simulated outside GIS environment, where it was applied to Embori and Mukogodo plot data using separate data sets for calibration and validation. Calibration was used to obtain input parameters for saturated hydraulic conductivity, cohesion and Manning’s roughness coefficients. Validation gave correlation coefficients of 0.907 and 0.840 for predictions of storm runoff and soil loss respectively at Embori; the corresponding values for bare soil plots at Mukogodo were 0.895 and 0.577. However, EUROSEM predicted runoff poorly (R2 = 0.570) and failed to predict soil loss at all the vegetated plots at Mukogodo. The model was applied to simulated vegetation covers of barley, maize, grass and forest for a 36.7 mm rainstorm at Embori. The simulated soil losses showed an exponential decrease with increasing cover. At a threshold cover of 70 percent, soil loss diminished to zero under grass and forest and decreased to a minimum value under barley and maize. These results support the USLE simulations, which showed that areas with more than 70 percent cover (such as forest) had a low erosion hazard, even with steep slopes and high rainfall erosivities. This research has demonstrated that GIS can be used with the USLE to assess and quantify erosion hazard, giving results that can be used for conservation planning. EUROSEM can be applied successfully to bare soil and cropland, but application to other land covers requires further investigation. Land cover and topography are the main factors controlling the spatial distribution of soil loss in the Upper Ewaso Ng’iro basin. Future conservation activities should be concentrated on the rangelands.
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