Browsing by Author "Alex, Wenaty"

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    Development of bioactive peptides erom whey by enzymatic hydrolysis
    (University of Reading, 2012-09) Alex, Wenaty
    I’hc major purpose of the project was to develop and apply an integrative process for the production of bioactivc peptides from the waste stream after adsorption of the acid whey by enzymatic hydrolysis, particularly the use of Protease N Amano. The study involved use of anionic resin in the adsorption process and Protease N Amano in the hydrolysis process at an enzyme: substrate ratio of 1/100 (wt/wt) added to the adsorbed protein in a batch membrane reactor at 45°C. Before hydrolysis the adsorbed protein was re solubilised using 30ml. of a lOmM (0.0IM) potassium phosphate buffer at pll 7. The resulting fractions were analysed for total protein by BCA assay and major whey proteins by IIP1.C. Results of the adsorption of waste stream show that 60.68% of 0-Lacloglobulin and 17.27% of a-I.aclalbumin were adsorbed by the anionic resin. Moreover, in the hydrolysis process the amount of protein hydrolyzed was found to increase with increase in hydrolysis time.