Development of bioactive peptides erom whey by enzymatic hydrolysis
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Date
2012-09
Authors
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Journal ISSN
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Publisher
University of Reading
Abstract
I’hc major purpose of the project was to develop and apply an integrative process for
the production of bioactivc peptides from the waste stream after adsorption of the
acid whey by enzymatic hydrolysis, particularly the use of Protease N Amano. The
study involved use of anionic resin in the adsorption process and Protease N Amano
in the hydrolysis process at an enzyme: substrate ratio of 1/100 (wt/wt) added to the
adsorbed protein in a batch membrane reactor at 45°C. Before hydrolysis the
adsorbed protein was re
solubilised using 30ml. of a lOmM (0.0IM) potassium
phosphate buffer at pll 7. The resulting fractions were analysed for total protein by
BCA assay and major whey proteins by IIP1.C. Results of the adsorption of waste
stream show that 60.68% of 0-Lacloglobulin and 17.27% of a-I.aclalbumin were
adsorbed by the anionic resin. Moreover, in the hydrolysis process the amount of
protein hydrolyzed was found to increase with increase in hydrolysis time.
Description
Master of Science in Food Technology: Quality Assurance
Keywords
Bioactive peptides erom, Enzymatic hydrolysis, Cow's milk, Waste stream