Articles, Conference and Workshop Papers Collection

Permanent URI for this collectionhttp://10.10.97.169:4000/handle/123456789/4447

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Detection of proteolysis in milk by pseudomonas fluorescens using urea PAGE method
(Macrothink Institute, 2017-12-28) Chove, Lucy Mlipano; Alistair, Grandison; Michael, Lewis
Proteolysis of milk during storage by two strains of Pseudomonas NCIMB 702085 (414) and NCIMB 701274 (416) was investigated using the Urea PAGE method. Pseudomonas fluorescens enzymes were also extracted and purified by dialysis before inoculation into UHT skim milk in an attempt to partially purify the enzyme. Results showed that dialysis removed some peptides and amino acids which would interfere with the assay procedure. The method also confirmed that Pseudomonas fluorescens NCIMB 701274 (416) was more proteolytic than Pseudomonas NCIMB 702085 (414). Thus, Urea PAGE is a useful method for monitoring proteolysis in milk by Pseudomonas fluorescens.
Effect of depth and distance of the borehole from the septic tank on the physico-chemical quality of water
(Macrothink Institute, 2017-12-28) Chove, Lucy Mlipano; Mongi, Richard; Chenge, Lawrence
A study to examine the effect of depth and distance of the boreholes from the septic tank on physico-chemical parameters of the borehole water consumed in Dar Es Salaam, Tanzania was conducted. Samples were collected from 48 boreholes in Ilala, Kinondoni and Temeke districts and subjected to pH, B.O.D (Biological Oxygen Demand), total hardness, copper and lead analyses in relation to safety and quality levels set by WHO and TBS. Analysis by R-statistics indicated that pH and B.O.D significantly (p<0.05) decreased as the depth of borehole increased, whereas total hardness was significantly (p<0.05) higher in very deep boreholes than the other heights. Total hardness and pH were not significantly (p>0.05) affected by distance between septic tank and borehole and the B.O.D levels were significantly higher in the borehole water situated near the septic tank than those which were far. Based on the physico-chemical characteristics of water, 25% of the samples did not comply with WHO and TBS specification limits while 75% complied, most of which had shallow depths and were situated near the septic tank. It may thus be concluded that both the distance and the depth between septic tank and borehole influenced physico-chemical characteristics of water.
Fruit consumption and storage practices among rural households in Chamwino district, Dodoma, Tanzania
(Academicjournals.org/AJFS, 2023-05) Chove, Lucy Mlipano; Mwanri, Akwilina Wendelin; Ngawembela, Mwanaisha Assim
Fruit consumption is still a challenge in many parts of Africa, and hence micronutrient deficiency continues to be a serious problem in the continent. This study was conducted between December 2017 and May 2018 in Chinoje and Mzula villages in Chamwino district, Dodoma to assess availability of fruit, consumption, storage practices and nutrient content. People responsible for food preparation were interviewed from 345 randomly selected households by using semi-structured and food frequency questionnaires. Multiple logistic regression model was used to determine the relationship between frequency of fruit consumption and household socio-economic features by using SPSS. Laboratory analysis was conducted to determine nutrient content of baobab, which was the most consumed fruit. Analysis of Variance (ANOVA) was used to determine if significant variations existed in the nutritional quality of baobab fruit by using SAS. Only 35% of the households consumed fruit daily, while the majority consumed fruit from one to three days in a week. Monthly income, household size and headship significantly affected fruit consumption at p<0.05. Most of the baobab fruits were stored in polypropylene sacks (77.4%), followed by plastic buckets (3.3%) and others as shelled fruit (18.4%). Significant losses in Vitamin C and total carotenoids were observed in baobab fruits that were stored in sacks. Storage of baobab fruit in plastic bucket is recommended for quality maintenance of nutrients.
Nutritional quality and functional properties of baobab (Adansonia digitata) pulp from Tanzania
(Canadian Center of Science and Education, 2016) Aluko, Angela; Kinyuru, John; Chove, Lucy Mlipano; Kahenya, Peter; Owino, Willis
Baobab (Adansonia digitata L.) is a majestic tree associated with human habitation in some of the semi-arid regions of Africa and establishes an enormous economic and nutritional importance to the rural residential districts. The fruit pulp is considered to be of high nutritional significance; particularly vitamin C and calcium, also possess antioxidant functions as well as high dietary fiber content. Although it is a potential fruit for improving local diets and livelihoods,this fruit is underutilized and its potential not yet fully acknowledged. This work was contracted with the aim of defining the nutritional quality and functional properties of baobab pulp harvested from some selected parts of Tanzania. Results indicated that the pulp from the three locations had moisture content which ranged between 9.16% to 10.30%, fat 0.46%-1.98%, ash 4.75%-5.21%, fiber 5.91%-9.65%, protein 3.23%-3.53%, carbohydrate 80.49%-85.19, vitamin C 169.74mg/100g-231.57mg/100g, beta-carotene 2.16 mg/100g-3.19mg/100g.Fructose 0.56±0.15-0.81±0.17g/100g, glucose 0.77±0.26-0.87±0.31g/100g and sucrose 0.75±0.25-0.84±0.29g/100g. The substantial differences (p≤ 0.05) between locations were observed in fat, crude fiber, carbohydrates, and fructose. Vitamin C, beta-carotene, protein, ash, moisture, sucrose and glucose showed no significance difference (p≤ 0.05) among locations. The functional properties included emulsification, foaming and gelling properties which ranged between 37.9-45.15%, 1.85-6.57% and 11-12% respectively and were significantly different (p≤ 0.05) among locations. The results show that baobab pulp has a good content of nutrients and functional properties which can be useful in food industries.
Preliminary study on the role of ionic calcium in gelation and proteolysis of UHT milk
(Macrothink Institute, 2017-12-28) Chove, Lucy Mlipano; Lewis, Michael
A study was conducted to determine the influence of ionic calcium on gelation and proteolysis in milk. Raw milk was heated to 142 °C for 2s followed by cooling to 5 °C to make UHT milk. Two sequestering agents, Trisodium citrate (TSC) and Sodium hexametaphosphate (SHMP) were added at 0.04 - 0.08% (w/v) to the processed UHT milk to reduce the ionic calcium levels. Proteolysis and gelation were induced by addition of trypsin (248 BAEE units), 0.03% (v/v) chymosin and 10 6 cfu/mL Pseudomonas fluorescens NCIMB 702085 (Ps. fl. 416) to UHT milk. Samples were stored at 25 °C for 21⁄2 weeks to monitor gelation and Ca 2+ and some were incubated at 37 °C for 2h to monitor proteolysis. SHMP reduced more Ca 2+ than TSC. Ca 2+ reduction was accompanied by an increase in pH, most evident with TSC at 25 °C. Gelation was not observed in samples inoculated with Ps. fl. 416 (with sequestering agents) even after 9 days of storage, suggesting the importance of calcium in gelation. Chymosin treated samples gelled on day 0, whereas other samples gelled after 4 days. Trypsin increased Ca 2+ to levels higher than originally present in control UHT skim milk. Although in the current study, proteolysis was higher in samples inoculated with Pseudomonas fluorescens 416, no clear relationship was established between proteolysis and gelation in UHT milk. This observation implies that proteolytic activity is not influenced by Ca 2+ . However, longer time study using sodium azide to prevent bacterial contamination would be required to confirm these findings.

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