Large induction of type III deiodinase expression after partial hepatectomy in the regenerating mouse and rat liver

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dc.contributor.authorKester, M. H. A.
dc.contributor.authorMatondo, R. B.
dc.contributor.authorToussaint, M. J. M.
dc.contributor.authorPunt, C. A.
dc.contributor.authorAarnio, A. M.
dc.contributor.authorDarras, V. M.
dc.contributor.authorEverts, M. E.
dc.contributor.authorBruin, A.
dc.contributor.authorVisser, T. J.
dc.date.accessioned2018-10-05T07:14:41Z
dc.date.available2018-10-05T07:14:41Z
dc.date.issued2008-09-11
dc.descriptionEndocrinology, 2009; 150(1): 540-545en_US
dc.description.abstractThe deiodinase types 1 (D1) and 2 (D2) catalyze the activation of T4 to T3, whereas type 3 deiodinase (D3) catalyzes the inactivation of T3 and T4. D3 plays a key role in ontrolling thyroid hormone bioavailability. It is highly expressed during fetal evelopment, but also in other processes with increased cell proliferation, e.g. in vascular tumors. Because tissue regeneration is dependent on cellular proliferation and is associated with activation of fetal genes, we evaluated deiodinase activities and mRNA expression in rat and mouse liver, as well as the local and systemic thyroid hormone status after partial hepatectomy (PH).Weobserved that in rats, D3 activity was increased 10-fold at 20 h and 3-fold at 48 h after PH; D3 mRNA expression was increased 3-fold at 20 h. The increase in D3 expression was associated with maximum 2- to 3-fold decreases of serum and liver T3 and T4 levels at 20 to 24 h after PH. In mice, D3 activity was increased 5-fold at 12 h, 8-fold at 24 h, 40-fold at 36 h, 15-fold at 48 h, and 7-fold at 72 h after PH. In correlation with this, D3 mRNA was highest (6-fold increase), and serum T3 and T4 were lowest at 36 h. Furthermore, as a measure for cell proliferation, 5-bromo-2_-deoxyuridine incorporation peaked at 20–24 h after PH in rats and at 36 h in mice. No significant effect on D1 activity or mRNA expression was found after PH. D2 activity was always undetectable. In conclusion, we found a large induction of hepatic D3 expression after PH that was correlated with an increased cellular proliferation and decreased serum and liver T3 and T4 levels. Our data suggest that D3 is important in the modulation of thyroid hormone levels in the regenerating liver, in which a decrease in cellular T3 permits an increase in proliferationen_US
dc.identifier.citationKester, M.H., Toussaint, M.J., Punt, C.A., Matondo, R., Aarnio, A.M., Darras, V.M., Everts, M.E., de Bruin, A., Visser, T.J. (2009). Large induction of type III deiodinase expression after partial hepatectomy in the regenerating mouse and rat liver. Endocrinology 150(1): 540-545en_US
dc.identifier.issn1945-7170
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/2607
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofseries10.1210/en.2008-0344;
dc.subjectDeiodinaseen_US
dc.subjectThyronineen_US
dc.subjectHepatectomyen_US
dc.subjectMouseen_US
dc.subjectRaten_US
dc.titleLarge induction of type III deiodinase expression after partial hepatectomy in the regenerating mouse and rat liveren_US
dc.typeArticleen_US
dc.urlhttps://academic.oup.com/endo/article-abstract/150/1/540/2455964en_US

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