Seroimmune responses to strategic vaccination in chickens against Newcastle disease using commercially available vaccines
| dc.contributor.author | Mengele,Isaac Joseph | |
| dc.date.accessioned | 2025-09-23T09:20:43Z | |
| dc.date.available | 2025-09-23T09:20:43Z | |
| dc.date.issued | 2014 | |
| dc.description | Dissertation | |
| dc.description.abstract | Evaluation of the Newcastle Disease (ND) antibody level after different vaccination strategies using 1-2 and La Sota vaccines was conducted on day old broiler chicks. Five groups for each type of vaccine, containing sixteen (16) randomly assigned chicks each were kept in none communicating pens. The chicks were accorded standard management procedures including ad libitum feeds and water as well as deworming and vaccination against fowl pox and infectious Bursa disease. At three weeks of age, chicks were wing tagged and vaccination strategies employed. Vaccination strategies employed for each vaccine type were 12.5%, 25% and 50%, the remained percentage was filled by in-contact chicks. Vaccination at 100% and no vaccination (0%) served as controls. Blood samples were collected before vaccination and then after every two weeks, five times post vaccination. Sera were prepared and tested for antibodies against Newcastle Disease virus using the standard Haemagglutination Inhibition (HI) procedure. At 12.5% vaccination strategy for 1-2, only 12.5% of the vaccinated chickens had protective antibodies level (HI titre results <log3 base 2) and none of the in contact chickens and the Geometric Mean Titre (GMT) of 1.13 was recorded and was not significantly different (p>0.05) with that of the negative control group. For La Sota vaccines at 12.5% strategy, 62.5% of the chickens had protective antibodies level and the GMT of 3.31 was recorded and was not significantly different (p>0.05) to the GMT of positive control group. At this strategy, La Sota vaccine has proved to do better than 1-2 vaccine in activating immune response in chickens. At 25% vaccination strategy, both 1-2 and La Sota vaccines showed that, 75% of the chickens had protective antibodies level. The GMT was 2.5 and 3.06 for 1-2 and La Sota respectively. These were significantly different (p<0.05) to GMT of the negative controls. | |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/7017 | |
| dc.language.iso | en | |
| dc.publisher | Sokoine University of Agriculture | |
| dc.subject | Seroimmune responses | |
| dc.subject | Strategic vaccination | |
| dc.subject | Newcastle disease | |
| dc.subject | Chickens | |
| dc.title | Seroimmune responses to strategic vaccination in chickens against Newcastle disease using commercially available vaccines | |
| dc.type | Thesis |