Occurrence and determination of antimicrobial resistant escherichia coli isolates in fish and vegetables as indicator organism of faecal contamination in Dar es salaam, Tanzania
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Hindawi International Journal of Microbiology
Abstract
Escherichia coli such as E. coli O157:H7, a non-sorbitol-fermenting (NSF) E. coli, is an essential human pathogen among other
common zoonotic pathogens carried by animals especially cattle. ,ey are discharged through cattle faeces into the environment.
With the increasing practice of urban farming, livestock manure is used as organic fertiliser in either fish ponds or vegetable
gardens. ,is practice increases the risk of transmission of such pathogens to humans. ,is study aimed at determining the
occurrence, antimicrobial resistance profiles, and genetic relatedness of E. coli isolates from manure, vegetables, and fish.
Microbiological standard methods were used to isolate and identify E. coli isolates from manure, vegetable, and fish samples.
Confirmed isolates on biochemical tests were tested for resistance against six antibiotics using the disc diffusion method.
Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) typing method was used to generate
fingerprints and determine the genetic relatedness of the E. coli isolates. Of 156 samples including 89 manure, 53 vegetables, and
16 fish, 36 (23.1%) samples were positive for E. coli from where a total of 48 E. coli different isolates were recovered that were
subjected to antimicrobial susceptibility testing and genetic relatedness. Of these isolates, 25 (52.1%) were resistant to at least one
antimicrobial agent and 12 (48.0%) showed multidrug resistance. ERIC-PCR profiles of E. coli isolates from manure, vegetables,
and fish showed genetic diversity with genetic relatedness ranging from 74.5% to 100%. Nine phylogenetic clusters (I–IX)
determined at 90% threshold level of genetic relatedness were identified among the isolates. ,is study determined the occurrence,
antimicrobial resistant patterns, and genetic diversity of antimicrobial-resistant E. coli isolates from different sources. ,is study
showed the potential of microbial health risk to humans through contamination, and hence, it is necessary to monitor and
improve husbandry practices in urban farming.
Description
Journal artical
Keywords
E. coli
Citation
https://doi.org/10.1155/2021/6633488