Inhibition of endosome-lysosome system acidification enhances porcine circovirus 2 infection of porcine epithelial cells

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dc.contributor.authorMisinzo, Gerald
dc.contributor.authorDelputte, Peter L
dc.contributor.authorNauwynck, Hans J
dc.date.accessioned2022-05-19T13:59:32Z
dc.date.available2022-05-19T13:59:32Z
dc.date.issued2008-02
dc.description.abstractRecently, Misinzo et al. (G. Misinzo, P. Meerts, M. Bublot, J. Mast, H. M. Weingartl, and H. J. Nauwynck, J. Gen. Virol. 86:2057-2068, 2005) reported that inhibiting endosome-lysosome system acidification reduced porcine circovirus 2 (PCV2) infection of monocytic 3D4/31 cells. The present study examined the effect of inhibiting endosome-lysosome system acidification in epithelial cells, since epithelial cells support PCV2 infection in vivo and are used in culturing PCV2 in vitro. Ammonium chloride (NH4Cl), chloroquine diphosphate (CQ), and monensin were used to inhibit endosome-lysosome system acidification. NH4Cl, CQ, or monensin increased PCV2 (Stoon-1010) infection by 726% ± 110%, 1,212% ± 34%, and 1,100% ± 179%, respectively, in porcine kidney (PK-15) cells; by 128% ± 7%, 158% ± 3%, and 142% ± 11% in swine kidney cells; by 160% ± 28%, 446% ± 50%, and 162% ± 56% in swine testicle (Sen_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/4192
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.subjectEndosome-lysosome system acidificationen_US
dc.subjectPorcine epithelial cellsen_US
dc.titleInhibition of endosome-lysosome system acidification enhances porcine circovirus 2 infection of porcine epithelial cellsen_US
dc.typeArticleen_US
dc.urldoi:10.1128/JVI.01229-07en_US

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