Reduction in genome size and DNA methylation alters plant and fruit development in tissue culture induced off-type banana (Musa spp.)

Simple item page
dc.contributor.authorMsogoya, T. J.
dc.contributor.authorGrout, B. W.
dc.contributor.authorRoberts, A.
dc.date.accessioned2019-07-26T15:41:33Z
dc.date.available2019-07-26T15:41:33Z
dc.date.issued2011
dc.descriptionJournal of Animal & Plant Sciences, 2011. Vol. 11, Issue 3: 1450-1456en_US
dc.description.abstractShoot tip in vitro micropropagation of banana (Musa AAA East Africa) cv.’ Uganda’ resulted in off-type plants with an altered plant and fruit development. This study was carried out to determine epigenetic mechanisms underlying the altered plant and fruit development of the in vitro derived off-type banana. The off-type banana was compared with in vitro micropropagation (MP) derived normal banana and conventionally propagated (CP) banana plants with no tissue culture history in their ancestry as controls. Plant development was estimated based on plant height, girth and number of days from planting to flowering. Fruit development was measured as the number of days from flowering to fruit maturation and senescence. Mechanisms underlying the altered plant and fruit development were determined based on global cytosine DNA methylation and 2C nuclear DNA content. Leaf cytosine DNA methylation and 2C nuclear DNA content were determined using reversed phase HPLC and flow cytometer, respectively. Results showed that the off-type banana was significantly (P < 0.05) taller and delayed to flower compared with the MP and CP derived banana. Similarly, the fruits of the off-type banana had significantly (P< 0.05) longer maturation, ripening and senescence periods than those of the true-totype fruits. The offtype derived plants had lower (P < 0.05) leaf global cytosine DNA methylation and 2C nuclear DNA amount compared with the MP and CP derived plants. These findings suggest that the altered plant and fruit development of the of-type banana are possibly under the control of reduced cytosine DNA methylation and nuclear DNA content. Further studies are required to identify specific genes which affect plant and fruit development upon undergoing demethylation.en_US
dc.identifier.issn2071 - 7024
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/2877
dc.language.isoenen_US
dc.publisherJAPSen_US
dc.subjectPlant developmenten_US
dc.subjectFruit developmenten_US
dc.subjectDNA methylationen_US
dc.subjectNuclear DNA contenten_US
dc.subjectIn vitro induced off-type bananaen_US
dc.titleReduction in genome size and DNA methylation alters plant and fruit development in tissue culture induced off-type banana (Musa spp.)en_US
dc.typeArticleen_US
dc.urlhttp://www.biosciences.elewa.org/JAPSen_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Genome size & methylation alter plant & fruit develop.pdf
Size:
176.63 KB
Format:
Adobe Portable Document Format
Description:
Main article
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.66 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Articles, Conference and Workshop Papers Collection