Microsatellite analysis of common bean (Phaseolus vulgaris L.) genotypes in Tanzania for diversity in seed iron and zinc micronutrients
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Date
2023-02-15
Journal Title
Journal ISSN
Volume Title
Publisher
Greener Journal of Plant breeding and Crop Science
Abstract
Common bean (Phaseolus vulgaris L.) grain has big dietary role in
supplying protein, energy, vitamins, dietary fibre and micronutrients for
millions of people worldwide. In Tanzania, reports have shown existence of
Fe and Zn micronutrient variabilities among the common bean genotypes.
Eighty-four (84) genotypes were collected to study such variability by seed
biochemical and leaf molecular marker analysis. The analyses revealed
significant (p < 0.001) genetic variability for seed Zn and Fe nutrient
contents. Highest seed Fe content was 118 ppm for genotype Kashiransoni
and for seed Zn was 51.81 ppm for Imponzo 5 genotype (both were
collected from NPGRC). With seven (7) SSR primers associated with Fe and
Zn traits, genetic diversity was evaluated. A marker BM154 scored PIC
value 0.967 mean while marker BM160 had a lowest PIC score of 0.899.
Using an estimated genetic similarity value, two main clusters with sub
clusters in the dendrogram were developed. To corroborate the UPGMA
analysis results, a Principal Coordinate Analysis (PCoA) was performed
and displayed outputs into scattered plot presentation. For Fe
micronutrient improvement purposes, Inula was proposed to be crossed
with Kashiransoni while Roba with Imponzo 5 for Zn micronutrient
improvement. Selection was based on both concentrations of nutrients for
each genotype and their genetic similarity distances.
Description
Journal article
Keywords
SSR, Common bean, Parent selection, Iron and Zinc micronutrients, Diversity