Green synthesis and characterization of silver nanoparticles from aqueous extract of harrisonia abyssinica fruits
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Date
2025-03
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Publisher
MDPI - Engineering Proceedings
Abstract
The synthesis of silver nanoparticles using phytochemical reducing agents is the
most preferred technique because of its low cost and environmental friendliness. Conse-
quently, there are several reports published on the synthesis of silver nanoparticles using
extracts from leaves, barks, roots, and fruit peels. However, information on the use of fruit
extracts for the synthesis of silver nanoparticles is limited. Thus, the green synthesis of sil-
ver nanoparticles (HAF-AgNPs) using phytochemicals extracted from Harrisonia abyssinica
fruit (HAF) is reported in the current study. The silver nanoparticles were synthesized
through chemical precipitation and characterized using UV-Vis spectrophotometry, trans-
mission electron microscopy (TEM), energy-dispersive X-ray (EDX), and X-ray diffraction
analysis (XRD). The findings showed that fabricated HAF-AgNPs were crystalline and
spherical, and exhibited a strong UV-absorption band at 420 nm. The appearance of a peak
at 3 keV in the EDX spectrum indicated metallic silver atoms in the fabricated nanoparticles.
The fabricated nanoparticles exhibited antibacterial activity against Gram-positive (Staphy-
lococcus aureus) and Gram-negative (Escherichia coli) bacterial strains. The antibacterial
activity was stronger for Staphylococcus aureus (MIC = 5 µg/mL) compared to Escherichia
coli (MIC = 10 µg/mL). The preliminary findings from the current study suggest that
the nanoparticles prepared from the extract could serve as a potential antibacterial agent
against Gram-positive and Gram-negative bacteria.
Description
Proceeding Paper
Keywords
Harrisonia abyssinica fruit, silver nanoparticle, green synthesis