Occurrence of Vibrio cholerae and Vibrio parahaemolyticus among milkfish farms in Zanzibar
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
ABSTRACT
Fishing is among the main economic activities of the people of
Zanzibar. Few fish dealers are transforming this sector into
mariculture. Among the farmed fish is milkfish. Diseases are
among the limiting factors in the development of the maricul-
ture industry. Among other zoonotic diseases, vibriosis is
caused by bacteria from the genus Vibrio. This study aimed
to establish the occurrence of Vibrio cholerae and Vibrio para-
haemolyticus among milkfish farms in Zanzibar. A total of 380
milkfish were sampled. Swabs were collected from gills, intes-
tine, and kidney of each sampled milkfish. Preliminary identifi-
cation of V. cholerae and V. parahaemolyticus was done by
biochemical tests. PCR was run on 16S rRNA, outer membrane
protein W, and collagenase genes to confirm Vibrio species,
V. cholerae, and V. parahaemolyticus respectively. Almost one-
third (32.1%) of all sampled milkfish were found to contain
targeted Vibrio; 18% and 29.5% of the sampled milkfish were
positive for V. cholerae and V. parahaemolyticus respectively.
Description
Journal article
Keywords
Milkfish, Prevalence, Vibrio Cholerae, Vibrio parahaemolyticus, Zanzibar
Citation
https://doi.org/10.1080/10454438.2019.1670317