Genetic characterization and antimicrobial susceptibility patterns of proteus mirabilis isolated from domestic rats in Arusha municipaltiy, Tanzania
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Date
2022
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Sokoine University of Agriculture
Abstract
The aim of this study was to genetically characterize and assess antimicrobial
susceptibility patterns of Proteus mirabilis isolates from rats cohabiting with human
dwellings. A cross sectional survey was conducted where a total of 139 rats were trapped
in houses and around peri domestic areas in selected wards in Arusha city. Following
euthanization, rats were identified to Genus/species level using morphological and
morphometric features. Deep intestinal swabs were ascetically obtained and pre enriched
in buffered peptone water prior laboratory analysis conventional culture methods and
biochemical methods were used for bacterial isolation. Molecular confirmation of the
isolates was done using the 16s ribosomal RNA PCR identification method.
Susceptibilities to Tetracycline (TE, 30μg), Trimethoprim sulfamethoxazole (SXT, 25
μg), Ciprofloxacin (CIP, 5μg), Cefotaxime (CTX,5μg), Ampicilin (AMP,10μg),
Azithromycin (AZM, 15μg) and Gentamycin (CN,10μg) was performed and resistance
genes (bla TEM, tetA, tetB, mphA, SHV, bla CTX-M, sul1 and sul2) were traced in each
isolate using PCR methods. Mixed rat species Rattus rattus (55.4%; n=77) Mus musculus
(15.8%; n=22) and Mastomys natalensis (28.8%; n=40) were captured. Proteus mirabilis
was isolated from 4 samples (2%) from Rattus rattus. All isolates were 100% similar to
P. mirabilis strains from NCBI. Constructed phylogenetic tree showed all P. mirabilis
isolated from this study were closely related to Tunisia isolates. Three isolates showed
MDR trait against Triomethoprim- sulfamethaxole, Azithromycin, and Ampicillin., all
isolates were resistance to Azithromycin, and Ampicillin, three were resistance to
Triomethoprim- sulfamethaxole, and intermediate to Tetracycline and all susceptible to
Ciprofloflaxcin, Gentamicin and Cefotaxime, PCR analysis showed the presence of TetA,
blaTEM, Sul1 and Sul2 resistant genes in all isolates. The study has shown that rats are
potential reservoirs of Proteus mirabilis with antimicrobial resistance trait that could be transmitted to the public and it’s the first study to isolate P. mirabilis from rats in
Tanzania with antimicrobial resistance trait.
Description
Dissertation
Keywords
Genetic characterization, Antimicrobial susceptibility patterns, Proteus mirabilis isolated, Domestic rats, Tanzania, Arusha municipality