Molecular characterization of infectious bursal disease virus recently detected in Dar es salaam, Tanzania

dc.contributor.authorBadi, P.
dc.date.accessioned2019-10-31T11:35:49Z
dc.date.available2019-10-31T11:35:49Z
dc.date.issued2018
dc.descriptionMasters Thesisen_US
dc.description.abstractInfectious bursal disease virus (IBDV) causes an acute and highly contagious immunosuppressive disease in young chickens aged 3 to 6 weeks. The molecular epidemiology of IBD virus causing severe disease in chickens in Tanzania has not been consistently studied. A cross-sectional study that involved collection of bursal of Fabricius from dead chicken following IBD outbreak(s) in Dar es Salaam was conducted. The laboratory analysis of samples was performed by reverse transcription polymerase chain reaction (RT-PCR), nucleotide sequencing, sequence alignment and phylogeny analysis targeting the VP2-hypervariable region (VP2-HVR). The findings of this study revealed that one out of eight samples (12.5%; n=1) was positive for VP2-HVR by RT-PCR and sequencing. A BLAST search of generated sequence indicated 96% nucleotide identity of the field isolate (TZ/DSM/2018) to the LUSC 47-2016 strain detected in chicken from Lusaka, Zambia. The TZ/DSM/2018 virus had conserved putative virulence marker amino acids at 222(A), 242(I), 256(I), 294(I) and 299(S) positions corresponding to very virulent IBDV feature, with unique amino acids at positions 263S and 338P. On phylogeny analysis, the TZ/DSM/2018 virus clustered in the same clade with the African VV-IBDV genotype. Taken together, this study has revealed the existence of the African VV-IBDV genotype in Dar es Salaam, which is genetically different from the vaccine isolate. Further studies are required to perform the in-depth genetic and antigenic characterization of circulating IBDV strains in Tanzania, so that a rational IBD control method can be recommended in the region.en_US
dc.description.sponsorshipPublic Health and Tropical Medicine grant WT104017MAen_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/2952
dc.language.isoenen_US
dc.publisherSokoine University of Agricultureen_US
dc.subjectMolecular characterizationen_US
dc.subjectBursal disease virusen_US
dc.subjectBursal diseaseen_US
dc.subjectDar es salaamen_US
dc.subjectTanzaniaen_US
dc.titleMolecular characterization of infectious bursal disease virus recently detected in Dar es salaam, Tanzaniaen_US
dc.typeThesisen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
PASKALINA BADI.pdf
Size:
532.68 KB
Format:
Adobe Portable Document Format

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.66 KB
Format:
Item-specific license agreed upon to submission
Description: