Browsing by Author "Rusagara, John Nzungize"

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    Molecular marker-assisted backcrossing of anthracnose resistance genes into common beans (Phaseolus vulgaris L.) varieties
    (Scientific Research Publishing Inc., 2021-05-26) Uwera, Annuarite; Musoni, Augustine; Assefa, Teshale; Rusagara, John Nzungize; Msolla, Susan Nchimbi
    Anthracnose, caused by Colletotrichum lindemuthianum, is a major disease of common bean and results in high yield loss. Due to the high degree of pathogenic variability of the fungus and the continual emergence of new races, genetic resistance in the host is not durable. Gene pyramiding using Marker Assisted Selection (MAS) is proposed as a viable approach to improve the durability of major genes conditioning resistance to anthracnose. In this study a common bean line Urugezi x AND 1062 susceptible to anthracnose but already improved for Pythium root rot resistance was improved for an thracnose resistance through a backcross breeding program. Genotypic selec tion was done in Rubilizi laboratory in Kigali, Rwanada whereas phenotypic selection was conducted in an anthracnose hotspot at Rwerere, a research Centre of the Rwanda Agricultural and Animal Resources Development Board (RAB). Analysis of variance for effect of bean varieties and anthracnose isolates on disease expression showed significant differences (p < 0.001) among varieties and isolates and for the interaction between isolates and va rieties. Developed BC2F1 plants were 41% of them resistant and 59% suscepti ble to anthracnose. However, the observed proportion of 26 resistants and 37 susceptible in BC2F1 plants didn’t fit the goodness of fit of the expected pro portion of 75 resistants to 25 susceptible. Only 41% of BC2F1 plants inherited the resistance genes and were phenotypically resistant. Presence of SCAR-markers, SAB3 and SBB14, in the developed resistant lines h suggested successful resistance transfer of anthracnose resistance genes.