Browsing by Author "Msoffe, P. L. M."

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Current manure management practices and hygiene aspects of urban and peri-urban livestock farming in Tanzania
(Livestock Research for Rural Development,, 2012) Lupindu, A. M.; Ngowi, H. A.; Dalsgaard, A.; Olsen, J. E.; Msoffe, P. L. M.
The recent expansion of urban and peri urban livestock farming has resulted in close contact between animals and humans, sometimes with adverse human health effects. A survey involving 119 cattle keeping households in urban and peri-urban settings of Morogoro, Tanzania revealed that manure management practices were different from traditional practices mainly due to lack of land. Manure was collected and conveyed by using tools by 94% of respondents, while others used water or bare hands. Seventy six percent of respondents collected manure from animal houses at least once a day, a feature that was associated with housing characteristics (p<0.05). Heaping was a common manure storage method although other cattle keepers directly spread manure on land. Manure was disposed of within residential area by 70% of respondents and this practice was associated with land area owned by or under control of the households (p<0.05). The current manure management practices did not protect either humans, animals or the environment against the risk of contamination with potential zoonotic pathogens and therefore there is a need for the formulation of guidelines on safe manure management practices.
Genetic diversity among nine free range local chicken ecotypes in Tanzania based on microsatelliteDNA polymorphisms
(2008-06) Msoffe, P. L. M.; Mtambo, M. M. A.; Minga, U. M.; juul-Madsen, H. R.; Gwakisa, P. S.
The genetic diversity among nine free-range local chicken ecoivpes identified in Tanzania was evaluated using 20 polymorphic microsatelliies markers. The ecotypes were named Ching'toekuie, Mbeya, Morogoro-medium, Kuchi, Singamagazi, N'zenzegere, Pemba, Tunga, and Unguja. A manual microsatellite typing procedure was adopted. Of the 20 microsaielliie loci, 16 were amplified by PCR and were highly polymorphic. Four to 15 alleles per locus and four to seven alleles per ecotype were detected. Gene diversity was venj high (62 to 79%). The shortest genetic distance as measured by Nei's standard genetic distance (Ds) was between Kuchi and Unguja ecotypes (Ds distance = 0.0671) while the White Leghorn and Unguja ecotype (Ds distance= 0.9149) presented the longest genetic distance. A Neighbour-loining dendogram based on Ds distance showed the local chicken ecotypes to assort into three clusters. The bootstrapping values within the dendogram ranged from 41 to 98%. These results indicate that the free-range local chicken ecotypes to exist in different genetic groups. The accurate genetic typing of this animal genetic resource is important in making rational decisions on the utilisation of the chickens for economical use without compromising the conservation of each unique ecotype adapted to a particular eco-climaiic zone.
Occurrence and characterization of Shiga toxin-producing Escherichia coli o157:h7 and other non-sorbitol–fermenting e. coli in cattle and humans in urban areas of Morogoro, Tanzania
(Mary Ann Liebert, Inc., 2014) Lupindu, A. M.; Olsen, J. E.; Ngowi, H. A.; Msoffe, P. L. M.; Mtambo, M. M.; Scheutz, F.; Dalsgaard, A.
Escherichia coli strains such as Shiga toxin–producing E. coli (STEC), enteropathogenic E. coli, enterotoxigenic, attaching, and effacing E. coli, and enteroinvasive E. coli cause diarrhea in humans. Although other serotypes exist, the most commonly reported STEC in outbreaks is O157:H7. A cross-sectional study was conducted to isolate and characterize non-sorbitol–fermenting (NSF) E. coli O157:H7 from urban and periurban livestock settings of Morogoro, Tanzania. Human stool, cattle feces, and soil and water samples were collected. Observations and questionnaire interview studies were used to gather information about cattle and manure management practices in the study area. E. coli were isolated on sorbitol MacConkey agar and characterized by conventional biochemical tests. Out of 1049 samples, 143 (13.7%) yielded NSF E. coli. Serological and antimicrobial tests and molecular typing were performed to NSF E. coli isolates. These procedures detected 10 (7%) pathogenic E. coli including STEC (n=7), enteropathogenic E. coli (EPEC) (n=2), and attaching and effacing E. coli (A/EEC) (n=1) strains. The STEC strains had the ability to produce VT1 and different VT2 toxin subtypes that caused cytopathic effects on Vero cells. The prevalence of STEC in cattle was 1.6%, out of which 0.9% was serotype O157:H7 and the overall prevalence of diarrheagenic E. coli in cattle was 2.2%. The serotypes O157:H7, O142:H34, O113:H21, O+:H-,O +:H16, and O25:H4 were identiﬁed. One ESBLproducing isolate showed the MLST type ST131. To our knowledge, this is the ﬁrst ﬁnding in Tanzania of this recently emerged worldwide pandemic clonal group, causing widespread antimicrobial-resistant infections, and adds knowledge of the geographical distribution of ST131. Cattle manure was indiscriminately deposited within residential areas, and there was direct contact between humans and cattle feces during manure handling. Cattle and manure management practices expose humans, animals, and the environment to pathogenic E. coli and other manure-borne pathogens. Therefore, there is a need to improve manure management practices in urban and periurban areas to prevent pathogen spread and associated human health risks.
Peste des Petits Ruminants (PPR) outbreak in southern, Tanzania
(RUFORUM, 2012) Muse, E. A.; Matondo, R. B.; Karimuribo, E. D.; Misinzo, G.; Mellau, L. S. B.; Msoffe, P. L. M.; Albano, M. O.; Gitao, G. C.
Peste des petits ruminants (PPR) was first confirmed in Tanzania in 2008, however description of clinical or pathological signs was not carried out although this is important to assist quick identification and reporting of PPR cases by both livestock keepers and field-based animal health workers. A study was therefore conducted to investigate and describe clinical signs and pathological lesions associated with suspected PPR cases in southern Tanzania. It involved history taking and clinical examination of suspected cases of 25 goats and 3 sheep. Post- mortem examination of some cases was performed followed by collection of specimens for histopathological examination. Swabs were also collected for confirmation of PPR by detecting ribonucleic acid using reverse transcription polymerase chain reaction (RT-PCR). Serum samples were analysed using competitive enzyme linked immunosorbent assay (cELISA). Severe depression, high fever, anorexia, muco-pulurent nasal discharge, erosive and necrotic stomatitis, mild diarrhoea and skin nodules were major signs suggestive of PPR. Post mortem examination showed evidence of pneumonia including lung congestion and consolidation. RT-PCR confirmed presence of the PPR virus in samples and serum antibodies showed seroprevalence of 31%.
Prevalence, antimicrobial resistance and risk factors for Thermophilic campylobacter infections in symptomatic and asymptomatic humans in Tanzania
(Blackwell Verlag Gmb, 2015) Komb, E. V. G.; Mdegela, R. H.; Msoffe, P. L. M.; Nielsen, L. N.; Ingmer, H.
The genus Campylobacter comprises members known to be a leading cause of foodborne gastrointestinal illness worldwide. A study was conducted to determine the epidemiology and antimicrobial resistance of Campylobacter in humans in Morogoro, Eastern Tanzania. Isolation of Campylobacter from stool specimens adopted the Cape Town protocol. Campylobacter isolates were preliminarily identiﬁed by conventional phenotypic tests and subsequently conﬁrmed by matrix-assisted laser desorption/ionization–time-of-ﬂight (MALDI-TOF) mass spectrometry and polymerase chain reaction. Antimicrobial resistance testing employed the disc diffusion method. A small proportion of the test isolates was also subjected to agar dilution method. Risk factors for human illness were determined in an unmatched case–control study. Thermophilic Campylobacter were isolated from 11.4% of the screened individuals (n = 1195). The agreement between PCR and MALDI-TOF was perfect (j = 1.0). Symptomatics and young individuals were infected with higher numbers than asymptomatic and adults, respectively. The majority (84.6%) of the isolates were C. jejuni and the remaining were C. coli. Isolates had highest resistance (95.6%) for colistin sulphate and lowest for ciproﬂoxacin (22.1%). The rates of resistance for other antibiotics (azithromycin, erythromycin, tetracycline, cephalothin, gentamycin, nalidixic acid, ampicillin, amoxycillin, norﬂoxacin, chloramphenicol) ranged from 44.1% to 89%. Comparison between disc diffusion and agar dilution methods indicated a good correlation, and the tests were in agreement to each other (j ≥ 0.75). Human illness was found to be associated with young age and consumption of chicken meat and pre-prepared salad. Our data indicate the presence of antibiotic-resistant thermophilic Campylobacter in humans in the study area. There is a need for routine investigation of the presence of the organisms in gastroenteritis aetiology, including determination of their antibiotic susceptibilities.
Seroprevalence and factors affecting canine monocytic ehrlichiosis and canine brucellosis in Tanzania
(Roavs, 2012) Muhairwa, A. P.; Msoffe, P. L. M.; Mtambo, M. M. A.; Mwakijungu, E. O.
A cross-sectional study was undertaken to determine the seroprevalence of Ehrlichia canis and Brucella canis in dogs in Morogoro Tanzania. The study was conducted between June and September 2010. A total of 100 randomly selected dogs were tested for the presence of Ehrlichia canis and Brucella canis antibodies using the Immunocomb ® dot-ELISA tests (Biogal, Israel). Epidemiological factors such as age, sex, breed, health status, body condition and tick infestation were studied. E. canis antibodies were detected in 25% (n=100) of the dogs. B. canis antibodies were not detected in any of the study dogs. The difference in seroprevalence between old and adult dogs was statistically significant (P<0.05). There was also a significant difference in seroprevalence between dogs in good and those in fair body conditions (P<0.05). Seropositivity to E. canis was not associated with the other epidemiological factors. This study provides the first serological evidence of E. canis infection but found no evidence of antibodies to B. canis in dogs in Morogoro. Canine ehrlichiosis was found to be a prevalent disease in Morogoro and calls for regular testing and treatment of clinical cases and tick control measures to protect dogs from E. canis infection. The study also points out the need for further investigation on the presence of canine brucellosis.