Browsing by Author "Mrope, S. A."

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    Isolation, identification and antibiogram of salmonella enterica from chicken eggs collected from selected wards in Morogoro municipality, Tanzania
    (Sokoine University of Agriculture, 2017) Mrope, S. A.
    Eggs provide protein to human. However, consumption of contaminated eggs with pathogenic Salmonellae (Salmonella enterica) imposes negative impacts to human health causing salmonellosis. The present study aimed at isolation, identification and antibiogram testing of Salmonella enterica from chicken eggs in Morogoro Municipality. A total of 570 raw, boiled, cracked, clean, and faecal soaked chicken eggs (120 local and 450 exotic) were collected from selected areas and examined from November to May 2016. Analysis was done by pooling samples using bacteriological standard methods where eleven (11) pathogenic Salmonellae (Salmonella. enterica) were confirmed to genus level by PCR using Spec primers pair for Salmonella invA gene amplified 284 bp of DNA fragment. Out of eleven (11) recovered Salmonellae 6 (20.0%) from egg contents and 5 (16.7%) from eggshell surfaces. The overall prevalence of isolated Salmonella enterica in eggs was 36.7% (95% CI: 0.199 – 0.561). It was worth noting that, local eggs were highly contaminated (20.0%) than exotic eggs (16.7%) whereas raw eggs were highly contaminated (26.7%) than boiled eggs (10%). Interestingly clean eggs were highly contaminated (20.0%) than faecal socked (13.3%). Statistical analysis between raw and boiled eggs showed significant differences. Moreover, eggs from Mlimani ward were highly contaminated 4 (13.33%) compared with eggs from other wards. Antibiogram test results showed sensitivity to: Ciprofloxacin, Chloramphenicol, Gentamicin, Tetracycline, Sulfamethaxole-Trimethoprim, Imepenem, Cefotaxime, Ceftriaxone and Caftazidime, and resistant only to Kanamycin. This study reports for the first time presence of pathogenic Salmonellae (Salmonella enterica) in chicken eggs and their antibiogram patterns, indicating that eggs are unwholesome for human consumption and population in Municipality is at risk for salmonellosis. Therefore, it is recommended that control measures from eggs production to table chain should be implemented by health sectors to prevent human/animal salmonellosis.
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    Salmonella enterica in raw and boiled eggs: call for comprehensive preventive measures throughout the production and processing chain
    (Tanzania Veterinary Journal, 2019) Mrope, S. A.; Lupindu, A. M.
    Eggs are good source of proteins to human. However, consumption of contaminated eggs may lead to ill-health. Salmonella enterica typifies one of such pathogens causing food poisoning in humans. The present study aimed at assessing the bacteriological quality of eggs from chicken in respect to Salmonella enterica. A total of 570 chicken eggs were collected and examined from November 2015 to May 2016 in Morogoro municipality. Salmonella was isolated from egg content and eggshell samples by conventional culture and biochemical methods. Salmonella enterica were confirmed to genus level by PCR targeting invA gene. Salmonella enterica were tested for resistance against common antimicrobial agents. Salmonella enterica was recovered from 11 out 30 sample pools, of which 6 (20.0%) from egg contents and 5 (16.7%) from eggshell surfaces. Eggs from local chicken breeds were highly contaminated (20.0%) than eggs from exotic chicken breeds (16.7%) whereas raw eggs were highly contaminated (26.7%) than boiled eggs (10%). Clean eggs were highly contaminated (20.0%) than faecal socked (13.3%) and cracked eggs (3.4%). Salmonella enterica were susceptible to Ciprofloxacin, Chloramphenicol, Gentamicin, Tetracycline, Sulfamethaxole-Trimethoprim, Imepenem, Cefotaxime, Ceftriaxone and Caftazidime, and resistant to Kanamycin. Presence of pathogenic Salmonellae in different forms of chicken eggs indicates that eggs are unwholesome for human consumption and control measures should be implemented throughout egg production chain to prevent human and animal salmonellosis.