Browsing by Author "Mdegela, R.H"
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Item Assessment of Antimicrobial Usage and Antimicrobial Residues in Broiler Chickens in Morogoro Municipality, Tanzania(Asian Network for Scientific Information, 2009) Nonga, H.E; Mariki, M; Karimuribo, E.D; Mdegela, R.HPresence of antimicrobial residues in broiler meat was determined in a cross-sectional study conducted in Morogoro, Tanzania between January and February, 2007. Twenty smallholder broiler chicken farmers were interviewed on the types of antimicrobials used and their awareness on withdrawal period. In addition, 70 broiler chicken liver samples were collected for qualitative antimicrobial residues analysis by use of two parallel tests; agar well diffusion and Delvotest SP ® assay. Ninety percent of the respondents frequently used tetracycline, amprolium, sulphonamides, trimethoprim, neomycine and flumequine to their chickens. Ninety percent of the respondents had knowledge on antimicrobial withdrawal period. However, 95% of farmers slaughtered their chicken before withdrawal period because were afraid of losses and were unaware of the effects of antimicrobial residues in humans. Laboratory results indicated that 70% (n = 14) of the farms were positive to antimicrobial residues. This shows a widespread misuse of antimicrobials by poultry farmers and reflecting lack of implementation of withdrawal times. It is stressed that stricter regulation for the use of antimicrobials in chicken and inspection of chicken for residues prior to marketing. However, poultry farmers need to be educated on the possible effects associated with use of food with antimicrobial residues.Item Assessment of Possible Sources of Endocrine Disruptors and Microbial Pathogens and their Impacts in the Lake Victoria Basin in the East African Region(2000) Mdegela, R.H; Mbuthia, P.G; Byarugaba, D.K; Mtenga, K; Kamundia, P.W.This study was conducted from August 2008 to May 2009 in Mwanza (Tanzania), Jinja (Uganda) and Kisumu, Homabay and Suba (Kenya) to establish the sources of pollutants in particular endocrine disruptors and microbial pathogens in water and fish. A sociological study was undertaken using qualitative and quantitative methods. Microbial contamination of water and fish in the lake was assessed in 14 water and 60 fish samples in Mwanza. In Jinja, assessment of microbial contamination was carried out in 100 water and 26 fish samples. A total of 80 Nile tilapia were collected from Kisumu, Homabay and Suba and examined for gross pathological lesions using standard necropsy techniques. Findings from the sociological studies indicated that, the knowledge and awareness on endocrine disruptors in the region is low. Pollutants that find access into the lake and have potential for disrupting the endocrine systems exist in all cities and originate from point and non-point sources. Human, industrial, agricultural, hospital and domestic wastes are the main sources of pollutants likely to contain endocrine disruptors. These pollutants are handled poorly and most of them are disposed off into the lake without treatment. In Mwanza, contamination of water with faecal material at various landing sites was observed in 85.7%, 78.6% and 35.7% of the samples determined using Total Viable Counts, Total Coliform Counts and Total Faecal Coliform Count. All water samples were negative for Salmonella spp, Vibrio spp, Cryptosporidia spp and Giardia spp. In Jinja, microbial contamination was observed in all sampling sites as determined by aerobic plate count (APC), Total Coliform Count (TCC) and Escherichia coli count. There was no significant difference in microbial load between sampling sites (P > 0.05). The gross pathological lesions were generalized hyperemia, liver changes (paleness, grey spots and hemorrhages), cystic urinary bladder, atrophied testis and kidneys, eye opacity, degenerated ovaries, grey patches on the stomach, clear cysts in the testis, deformed dorsal fin and ulcers on the lateral side of the body. Further studies are underway to confirm the likely causes of the observed lesions and the impact of the observed pollutants and microbial pathogens.Item Awareness, knowledge and practice of pastoralists and agro- pastoralists towards livestock diseases affecting domestic animals in Arusha, Manyara and Morogoro Regions, Tanzania(2013) Chengula, A; Mdegela, R.H; Kasanga, C.JThe study was carried out to assess pastoralists and agro-pastoralists awareness, knowledge and practice in various livestock diseases affecting domestic animals in Arusha Manyara and Morogoro regions in Tanzania. Closed- and open-ended questionnaires, focus group discussions and in-depth interview techniques were employed. Diseases, drought, lack of dipping tanks, insufficient of livestock experts and drugs are the main constraints in the livestock keeping community in the study area. Nineteen diseases have been reported to affect their animals at one time or the other. East Coast fever (ECF, 79.7%), Contagious Caprine Pleuropneumonia (CCPP, 60.8%) and Trypanosomosis (50%) have been reported by more than 50% of pastoralists that they affect their animals. ECF and CCPP seem to be the leading diseases with great impact to the pastoralists by causing high mortality rates. Rift valley fever (RVF) and anthrax have been reported by majority to be diseases which appear in form of outbreak in their area. Diseases reported here are said to be controlled primarily by treating with various drugs and Oxytetracycline being a common drug of choice for most unknown diseases. Other control methods include vaccination and deworming, dipping and spray of animals using acariceides. Livestock experts have been reported to play little role in controlling common livestock diseases as majority of livestock keepers tends to treat their animals. Veterinary experts seem to be important during outbreak of diseases or for unknown diseases killing many animals. Eating of dead and improperly cooked meat together with un-boiled milk was found to be common in the pastoral community. This could lead to the spread (if any) of zoonotic diseases easily. Livestock keeping community is aware of most of common diseases circulating in their area but the way they practice to control leads to failure of control of those diseases at individual and national level. Devising a mechanism to educate them so that they know how to handle some common and reporting outbreak diseases such as use of trained community animal health workers (CAHWs) will help control livestock diseases in Tanzania.Item Bioaccumulation and public health implications of trace metals in edible tissues of the crustaceans Scylla serrata and Penaeus monodon from the Tanzanian coast.(Springer International Publishing, 2017) Rumisha, C; Leermakers, M; Mdegela, R.H; Kochzius, M; Elskens, MThe coastal population in East Africa is grow- ing rapidly but sewage treatment and recycling facilities in major cities and towns are poorly developed. Since estuarine mangroves are the main hotspots for pollut- ants, there is a potential for contaminants to accumulate in edible fauna and threaten public health. This study analysed trace metals in muscle tissues of the giant mud crabs (Scylla serrata) and the giant tiger prawns (Penaeus monodon) from the Tanzanian coast, in order to determine the extent of bioaccumulation and public health risks. A total of 180 samples of muscle tissues of S. serrata and 80 of P. monodon were collected from nine sites along the coast. Both species showed high levels of trace metals in the wet season and significant bioaccumulation of As, Cu and Zn. Due to their burrowing and feeding habits, mud crabs were more contaminated compared to tiger prawns sampled from the same sites. Apart from that, the measured levels of Cd, Cr and Pb did not exceed maximum limits for human consumption. Based on the current trend of fish consumption in Tanzania (7.7 kg/person/year), the mea- sured elements (As, Cd, Co, Cu, Mn, Pb and Zn) are not likely to present health risks to shellfish consumers. Nevertheless, potential risks of As and Cu cannot be ruled out if the average per capita consumption is exceeded. This calls for strengthened waste manage- ment systems and pollution control measuresItem Calf health and management in smallholder dairy farms in Tanzania(Springer Verlag (Germany), 2010-06-25) Chang’a, J.S; Mdegela, R.H; Ryoba, H; Løken, T; Reksen, OAbstract Smallholder farmers’ knowledge and practice of dairy calf management on 129 farms with calves less than 10 months of age in Southeastern and Southern Highland areas of Tanzania was assessed. The method of study included both a farm visit and completion of a questionnaire. Most of the farmers were female, with a primary level of education, and majority kept 1–3 milking cows thatyielded 6–10 l milk/cow/day. Most of the calves were fed milk using a residual calf suckling system. Weaning age was 3–8 months. Overall, the body condition of the calves was poor, ranged from 1 to 2.5 with a mode of 2. The majority of the farmers believed that helminthosis was the most common disease condition affecting the calves; diarrhea was ranked as the second. Calf death was reported by 20% of the farmers to have occurred in their herd lasting the 2 years prior to the study. Calf body condition score was related to body weight for calves younger than 9 weeks, andolder than 23 weeks of age, whereas no such relationship existed in the age group 9 to 23 weeks. The sex distribution was skewed with less male calves being older than 23 weeks. We hypothesize that male calves experience inferior management compared with female calves. This studydemonstrates a low level of knowledge on, and poor practices of calf management among the surveyed farmers that suggest the need for educational intervention.Item Factors associated with body weight attainment in calves on smallholder dairy farms in Tanzania(2012-03-09) Chang’a, J.S; Løken, T; Mdegela, R.H; Reksen, OIn order to investigate associations between individual calf parameters and/or herd management practices and dairy calf body weight (BW) attainment in smallholder farms in Tanzania, a prospective study was carried out at 121 farms in Mvomero and Njombe districts and included 156 crossbred dairy calves. Information on management factors and sources of calf drinking water were collected by personal observation and interview during four visits. At each visit, BW assessments were conducted by the same veterinarian and blood samples collected. The blood samples were ana- lyzed for hematological parameters, total blood protein, glucose, and serum phosphorus and urea. Low BW was associ- ated with smaller amount of milk fed to calves, early weaning, birth during the wet season, and well/tap water as the main water source (P < 0.05). Low blood total protein, high urea, low phosphorus and low hemoglobin concentrations were also associated with retarded growth. These findings identified and quantified some critical factors that can guide farmers in improving calf performance on smallholder dairy farms in Tanzania. To improve calf BW, the protein and mineral fraction of the roughage should be increased and water supply should be improved. In conclusion, appropriate changes in milk feeding routines and breeding management would also be expected to improve productivity.Item Knowledge, attitudes and practices regarding porcine cysticercosis control among smallholder pig farmers in Kongwa and Songwe districts, Tanzania: a cross-sectional study(Elsevier, 2023) Wilson, C; Nonga, H.E; Mdegela, R.H; Churi, A.J; Mkupasi, E.M; Winkler, A.S; Ngowi, H.ATaenia solium taeniasis/cysticercosis (TSTC) is a parasitic zoonotic disease that is endemic in several developing countries, causing serious public health and economic impacts. A cross-sectional study was conducted to assess knowledge, attitudes and practices (KAP) related to porcine cysticercosis (PCC) transmission, prevention and control among smallholder pig farmers in Kongwa and Songwe Districts in Tanzania. A semi-structured ques tionnaire was administered to 692 smallholder pig farmers from randomly selected households. STATA software version 17 was used to analyse quantitative data, summarize farmers’ KAP about PCC and calculate performance scores. Nearly half (42%) of the respondents had little knowledge regarding PCC, only 17% of the respondents had good practices towards prevention/control of PCC and 72% had a positive attitude towards PCC- prevention/ control measures. The majority (73%) of smallholder pig farmers admitted deworming their pigs regularly, whereas 76% reported deworming themselves and their family members regularly. Albendazole and ivermectin are the most commonly used medications for deworming people and pigs, respectively. According to the findings, the majority of smallholder pig farmers in Kongwa and Songwe Districts showed a good attitude towards PCC prevention/control measures but had limited knowledge of the PCC life cycle and control. In addition, only one in five farmers was engaged in good practices. The findings revealed further that farmers are engaged in risky behaviours that aid the spread and perpetuation of the T. solium parasite in the study area. It is recommended that farmers should be given proper health education on the T. solium transmission cycle and preventive/control practices to limit PCC transmission.Item Occurrence of staphylococcus aureus in fresh Indian mackerel fish(Tanzania Veterinary Journal, 2019) Ali, F. S; Lupindu, A.M; Mdegela, R.H; Mmoch, A.JFish provide important protein to human population. The procedures to preserve and maintain quality of fish from fishing until consumption can play a role in contamination with pathogens. Consumption of contaminated sea food products such as fish may lead to food poisoning. Knowledge about the spectrum of fish bacterial contaminants may assist in prevention of contamination and control food poisoning incidences. The present study aimed at characterizing and estimating prevalence of Staphylococcus aureus in fresh Indian Mackerel Fish (Rastrelliger kanagurta) from landing sites in Unguja Island. A total of 400 Indian Mackerel Fish were collected from landing sites in Unguja Island and from each fish two samples, skin swab and muscle, were collected. The primary culture was obtained from Mannitol salt agar, Nutrient and Blood agar followed by Gram staining, catalase coagulase tests. PCR targeting 16S rRNA, nuc, mecA, pvl, spa and enterotoxin genes was run to genetically characterize isolates and identify S. aureus. The result indicates that there was growth of bacteria in 359 (89.75%) fish skin swabs and 102 (25.5%) in fish muscle samples. Based on biochemical tests, 27 isolates (6.75%) were confirmed to be Staphylococcus bacteria. Of the 27 isolates, seven (1.75%) were confirmed S. aureus based on PCR. All 27 isolates confirmed to be positive in 16Sr RNA gene, two isolates demonstrated mecA gene and one had SEB and SEC. Detection of S. aureus in fresh Indian Mackerel Fish at landing sites poses a contamination risk to other critical points along the value chain and threatens public health.Item Preliminary Observation on accessibility and utilization of water in selected villages in Dodoma rural and Bagamoyo districts, Tanzania(WaterNet/Warfsa, 2003) Kusiluka, L.J.M; Mlozi, M.R.S; Munishi, P.K.T; Karimuribo, E.D; Luoga, E.J; Mdegela, R.H; Kambarage, D.MItem Prenatal exposure to persistent organic pollutants in Northern Tanzania and their distribution between breast milk, maternal blood, placenta and cord blood. Environmental Research(ELSEVIER, 2019) Müller, M.H.B; Polder, A; Brynildsrud, O.B; Grønnestad, R; Karimi, M; Lie, E; Manyilizu, W.B; Mdegela, R.H; Mokiti, F; Murtadha, M; Nonga, H.E; Skaare, J.U; Solhaug, A; Lyche, J.LHuman exposure to persistent organic pollutants (POPs) begins during pregnancy and may cause adverse health effects in the fetus or later in life. The present study aimed to assess prenatal POPs exposure to Tanzanian infants and evaluate the distribution of POPs between breast milk, maternal blood, placenta and cord blood. For assessment of prenatal exposure, 48 maternal blood samples from Mount Meru Regional Referral Hospital (MMRRH), Arusha Tanzania, were analyzed for organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), brominated flame retardants (BFRs), dioxin-like (DL) activity and perfluorinated alkyl substances (PFASs). For evaluation of POPs distribution between maternal/infant compartments, breast milk, placenta and cord blood corresponding to the maternal blood were analyzed for OCPs, PCBs and BFRs. In maternal blood, p,p ́- DDE was detected in 100% of the samples ranging between 29 and 1890 ng/g lipid weight (lw). PCB-153 was the only PCB detected in maternal blood, with detection rate of 29% and concentrations up to 116 ng/g lw. BDE-47 was detected in 65% of the maternal blood samples, ranging betweenItem Prevalence of clinical and subclinical mastitis and quality of milk on smallholder dairy farms in Tanzania(2009) Mdegela, R.H; Karimuribo, E. D; Ryoba, R; Phiri, E.J; Løkenc, T; Mtengeti, E; Urio, N.AA cross sectional study was conducted during October and November 2006 on 69 smallholder dairy farms with lactating cows in Mvomero and Njombe districts Tanzania, to determine the prevalence of mastitis and to assess the milk quality on the study farms. Clinical mastitis was investigated using clinical changes of udder and milk at animal level. Cow-side California Mastitis Test (CMT) and microbiological cultures were used to assess subclinical mastitis at quarter level. Milk quality was determined on bulk milk samples at herd level using alcohol and acidity tests, butter fat content, total solids, ash content as well as Delvotest® for antimicrobial residues. Overall prevalence of clinical mastitis at herd level in both districts was 21.7 % (n = 69). Based on CMT, prevalence of subclinical mastitis at animal level was 51.6 %(n = 91). Prevalence of bacterial isolates at animal level was 35.2% (n = 91) while for fungal it was 16.7 % (n = 90). Based on CMT results, prevalence of subclinical mastitis at quarter level was 30 % (n = 353), while for bacteria and fungi it was 16%and 6%respectively. Contamination of milk with antimicrobial residues was 4.5%(n= 67). The milk quality parameters for most of the milk samples were within acceptable levels. Findings in this study have demonstrated high prevalence of subclinical mastitis that may contribute to low productivity of dairy cattle in both districts. About 20%ofCMTsubclinical cases had no involvement of microbial pathogens that suggested the need for minimal interventions with antimicrobial agents. These findings call for use of udder disinfectants and improved milking hygiene as intervention strategies to control mastitis on the smallholder dairy farms in Tanzania.Item Socio-economic impact of Rift Valley fever to pastoralists and agro pastoralists in Arusha, Manyara and Morogoro regions in Tanzania(2013) Chengula, A.A; Mdegela, R.H; Kasanga, CRift Valley fever (RVF) is a viral notifiable zoonotic disease primarily of domestic ruminants that causes significant socio-economic impacts. Using the 2006–07 outbreak cases, this study aimed to establish the socio-economic impact of RVF and assessing knowledge, attitude and practice of livestock keepers towards controlling RVF in selected areas of Tanzania. Data were collected in Arusha, Manyara and Morogoro regions using questionnaires, focus group discussions and in-depth interviews with key informants. Results indicate that there was little knowledge on disease (all clinical signs scored <50%) and the difference between the three regions was statistically significant (P = 0.00459). Socio-economic impacts of RVF shown by this study included; animal and human deaths, disruption of livestock market chains, inability of pastoralists to achieve their daily demands, inability to obtain protein leading to malnutrition and monetary loss at individual and national level during control of the disease. These findings have demonstrated low knowledge of the community on RVF, thus, more education and engagement is needed in order to develop more effective and efficient control strategies.