Browsing by Author "Kessy, B. M."
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Item Antimicrobial resistance and genotypic diversity of Campylobacter isolated from pigs, dairy, and beef cattle in Tanzania(Frontiers in Microbiology, 2015-11-12) Kashoma, I. P.; Kassem, I. I.; Kumar, A.; Kessy, B. M.; Gebreyes, W.; Kazwala, R. R.; Rajashekara, G.Foodborne Campylobacter infections pose a serious threat to public health worldwide. However, the occurrence and characteristics of Campylobacter in food animals and products remain largely unknown in Tanzania. The objective of this study was to determine the revalence, antibiotic resistance, and genetic profiles (sequence types, STs) of Campylobacter isolated from feces of pigs and dairy and beef cattle in Tanzania. Overall, 259 (∼30%) of 864 samples were positive for Campylobacter spp, which were detected in 32.5, 35.4, and 19.6% of the pig, dairy, and beef cattle samples, respectively. Multiplex PCR analysis identified 4.5 and 29.3% of the Campylobacter isolates as C. coli and C. jejuni, respectively. The majority (91.9%) of the isolates from pig samples were identified as C. coli, while C. jejuni accounted for 65.5% of the isolates from cattle. Antimicrobial susceptibility testing using the disk diffusion assay and the broth microdilution method revealed resistance to: ampicillin (Amp) (70.3% and 75.7%, respectively), gentamicin (Gen) (1.8% and 12.6%), streptomycin (Str) (65.8 and 74.8%), erythromycin (Ery) (41.4 and 48.7%), tetracycline (Tet) (18.9 and 23.4%), and ciprofloxacin (Cip) (14.4 and 7.2%). Resistance to nalidixic acid (Nal) (39.6%), azithromycin (Azm) (13.5%), and chloramphenicol (Chl) (4.5%) was determined using the disk diffusion assay only, while resistance to tylosin (Tyl) (38.7%) was quantified using the broth microdilution method. Multilocus sequence typing of 111 Campylobacter isolates resulted in the identification of 48 STs (26 C. jejuni and 22 C. coli) of which seven were novel (six C. jejuni and one C. coli). Taken together, this study revealed the high prevalence, genetic diversity and antimicrobial resistance of Campylobacter in important food animals in Tanzania, which highlights the urgent need for the surveillance and control of Campylobacter in this country.Item Blood metabolites and their relatioriship to dairy cattle productive and reproductive performance in Smallholder farms in Morogoro, Tanzania(2002) Nkya, R .; Kessy, B. M.; Aboud, A.A.Sixty-four dairy cows (B taums x B indicus crosses) belonging to 40 peri-urban smallholder farms were investigated for relationship of their blood metabolites, body condition score (BCS) and body weight to milk yield and reproductive performance. Feed availability and quality were monitored monthly. Plasma concentrations of selected metabolites [albumin globulin beta-hydroxybutyrate (BHB) inorganic phosphorus urea] and packed cell volume (PCV) were measured at one month before calving at 30 days and 60-90 days postpartum (DPP). Milk yield BCS and body weight were taken once a month. The reproductive status was monitored by measuring milk progesterone (P4) concentrations and rectal palpation. Average daily food intake was 98=0.3 kg DM/day. Cows calved in good BCS (4-6) had mean milk yield between 6. 4±0. 44 and 7.3±0.66 L day in the first 90 days of lactation and delayed calving to conception interval (126.1 ± 6. 3 days) There was no relationship between apparent feeding level and therefore change in body weight and BCS with milk yield and reproductive performance (P>0.0.5). The mean body weight and BCS dropped within one month after calving which gradually recovered within the second month postpartum. A large proportion of cows (22-38%) had low PCV values (<27%) before calving to 60-90. DPP an indication of anaemia possibly due to low feed intake and probably diseases. Blood metabolite values remained more or less constant before calving and during the first 60-90. DPP 24% of cows had high BHB values (1 nmol L) before calving indicating a negative energy balance in late gestation. Globulin levels were high (50. g L) in 20% of the cows during the 60-90 DPP period suggesting probably an inflammatory reaction within the body. The differences between metabolite concentration among animals were small and they were not significantly related to productive and reproductive performance (P<.0.5). The use or metabolic profile testing identified under-nutrition as the potential constraint to productivity in dairy cows in smallholder farms in Morogoro.Item Productive and reproductive performance of Friesian cows at Kitulo livestock multiplication unit, Tanzania(Tanzania Veterinary Journal, 2015-02-12) Kashoma, I. P.; Mwingira, S. M.; Werema, C. W.; Kessy, B. M.Retrospective data were collected to assess reproductive and productive performance of Friesian dairy cows reared at Kitulo Livestock Multiplication Unit, Tanzania. Records of 314 cows within consecutive five productive periods; 2009 - 2014 were analyzed using Statistical Package for SocialScience (SPSS). The overall mean age at first calving (AFC), calving interval (CI), days open (DO), number of services per conception (NSPC), lactation length (LL) and total lactation milk yield (TLMY) were 1151.72±9.63 days, 404.57±1.54 days, 121.82±1.24 days,1.83±0.24, 306.54±5.53 days and 5,042.12±23.73 kg, respectively. CI differed significantly (P < 0.05) as it decreased with increasing parity but did not affect the TLMY. Number of services per conception positively correlated with DO and CI but negatively correlated with parity. Total lactation milk yield was significantly (P < 0.05) influenced by parity and lactation length, but TLMY had no significant (P > 0.05) effect on the average number of services per conception. It is concluded that reproductive and productive performance of Friesian dairy cows in the present study were low to moderate so it is required to improve management practices at the farm for better reproductive and productive performance.