Browsing by Author "Issa-Zacharia, A."
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Item Efficacy of anaerobic methane fermentation system on Shochu waste treatment(J SASJ, 2009) Issa-Zacharia, A.; Morita, K.; Kamitani, Y.This study aimed at evaluating the efficacy of anaerobic methane fermentation on Shochu wastewater treatment and collecting the performance data for this system. The performance data studied include Chemical oxygen demand (CODcr) removal efficiency, methane production rate (MPR) and biogas production rate (BPR). Shochu wastewater contained 43,000mg/l and 36,800mg/l as total CODcr and suspended solids, respectively. Approximately CODcr removal efficiency of 98% could be achieved after 120h of fermentation in a batch reactor. The optimal biogas production was attained at the shochu wastewater to seed ratio of 1:4 (v/v). Undiluted shochu wastewater sample gave the best results indicating that its dilution is unnecessary. Initial material pH value of 6.5 to 7.5 for the shochu wastewater-seed mixture was effective and the pH outside this range resulted in reduced biogas production. Methane (CH4) content in biogas was on average 65.5% regardless of the initial pH value, with exception of pH 5.5 where no CH4 gas was detected. The BPR of 0.95l/ld and MPR of 0.43l/ld were realized with undiluted shochu wastewater at 1:4 (v/v) shochu wastewater to seed ratio. A large fraction of organic matter was effectively degraded.Item In vitro inactivation of Escherichia coli, Staphylococcus aureus and Salmonella spp. using slightly acidic electrolyzed water(The Society for Biotechnology, 2010) Issa-Zacharia, A.; Kamitani, Y; Tiisekwa, A.; Morita, K.; Iwasaki, K.In the current study, the effectiveness of slightly acidic electrolyzed water (SAEW) on an in vitro inactivation of Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Salmonella spp. was evaluated and compared with other sanitizers. SAEW (pH 5.6, 23 mg/l available chlorine concentration; ACC; and 940 mV oxidation reduction potential; ORP) was generated by electrolysis of dilute solution of HCl (2%) in a chamber of a non-membrane electrolytic cell. One milliliter of bacteria suspension (ca. 10–11 log 10 CFU/ml) was mixed with 9 ml of SAEW, strong acidic electrolyzed water (StAEW; ca. 50 mg/l ACC), sodium hypochlorite solution (NaOCl; ca.120 mg/l ACC) and distilled water (DW) as control and treated for 60 s. SAEW effectively reduced the population of E. coli, S. aureus and Salmonella spp. by 5.1, 4.8, and 5.2 log 10 CFU/ml. Although, ACC of SAEW was more than 5 times lower than that of NaOCl solution, they showed no significant bactericidal difference (p N 0.05). However, the bactericidal effect of StAEW was significantly higher (p b 0.05) than SAEW and NaOCl solution in all cases. When tested with each individual test solution, E. coli, S. aureus and Salmonella spp. reductions were not significantly different (p N 0.05). These findings indicate that SAEW with low available chlorine concentration can equally inactivate E. coli, S. aureus and Salmonella spp. as NaOCl solution and therefore SAEW shows a high potential of application in agriculture and food industry as an environmentally friendly disinfection agent.Item Persistent child malnutrition in Tanzania: risks associated with traditional complementary foods (A review)(2010-11) Muhimbula, H. S.; Issa-Zacharia, A.Despite numerous nutritional interventions that have taken place in Tanzania, the Country still experiences a high rate of child malnutrition. Millions of children suffer from one or more forms of malnutrition resulting in stunting, underweight, wasting and anemia. The growth of children often declines with the introduction of complementary foods around the age of 6 months and continues to decline up to 24 months that have greater implications for health during adult-hood. Poor breastfeeding and child feeding practices augmented by very early introduction of nutritionally inadequate and contaminated complementary foods are major factors contributing to persistent child malnutrition in Tanzania. These complementary foods comprise mainly cereal-based porridges with little or no vegetables and often lacking animal proteins. The promotion of exclusive breastfeeding during the first 6 months and appropriate feeding practices has been the integral parts of the intervention processes taking place in Tanzania. Food safety education is a critical prerequisite in the child health programs and is a cost effective intervention with long term positive impacts. This paper reviews the persistent child malnutrition in Tanzania and the causative factors to highlight risks associated with use of complimentary.Item A review of microbiological safety of fruits and vegetables and the introduction of electrolyzed water as an alternative to sodium hypochlorite solution(Academic Journals, 2010) Issa-Zacharia, A.; Kamitani, Y.; Muhimbula, H. S.; Ndabikunze, B. K.Over the past few years, food safety has become and continues to be the number one public concern. Considerable progress to strengthen food safety systems has been achieved in many countries, highlighting the opportunities to reduce and prevent food-borne disease. However, unacceptable rates of food-borne illness still remain and new hazards continue to enter the food supply chain. Contaminations in food and agricultural products may occur in every stage of the food supply chain, from the field to the table, that is production, harvesting, processing, storage and distribution, calling for proper decontamination and insuring food safety at each of these stages using an effective antimicrobial agent. Several commercial products are available for this purpose, however, most of available products are seriously hindered by a number of work and environmental safety limitations calling for the development of a new product which is both safe for environment and workers. In this accord, the use of acidic electrolyzed water (AEW), a new concept developed in Japan, which is now gaining popularity in other countries has been introduced. The principle behind its sterilizing effect is still explored, but it has shown to have strong and significant bactericidal and virucidal and moderate fungicidal properties. Some studies have been carried out in Japan, China, Korea, Canada, Europe and the USA on its pre- and post-harvest application in the field of food processing. This review provides an overview of microbiological safety of food and agricultural produces, points out the burdens of food borne diseases; highlights the drawbacks of currently employed sanitizers and introduces electrolyzed water as a novel non-thermal food sanitizer with potential of application in agriculture and food industry.Item Stability of slightly acidic electrolyzed water on storage and its microbial inactivation effectiveness on the aerobic microflora present on intact spinach (Spinacia oleracea L.) leaves(J. SAS, 2009) Issa-Zacharia, A.; Morita, K.; Kamitani, Y.The stability of Slightly Acidic Electrolyzed Water (SAEW) on storage was evaluated. SAEW samples of known initial Oxidation Reduction Potential (ORP, mV), pH and available chlorine concentration (ACC, mg/L) were stored at 25℃ in different containers and changes of ORP, pH and ACC were monitored daily for a period of 10 days. ORP, pH and ACC were respectively maintained at 900-1000mV, 5-6.5 and 10-30mg/L. SAEW was further evaluated for its microbial inactivation effectiveness on the aerobic microflora present on spinach (Spinacia oleracea L.) expressed as aerobic plate count (APC) and was compared to Sodium hypochlorite (NaOCl) solution. The decontamination was done by dipping spinach samples with or without a pre-washing step into treatment solution for 5min. SAEW (pH 5.5, 25mg/L ACC) achieved a significantly higher microbial reduction than NaOCl solution (pH 9.9, 103mg/L ACC). While pre-washing of spinach in running tap water for 5min increased the microbial reduction by SAEW from 1.3 logo colony forming units (CFU)/g to more than 2 log10 CFU/g at 5min contact time, increasing exposure time did not significantly affect its antimicrobial effectiveness on spinach aerobic microflora.