Sokoine University of Agriculture

Identification of microcystins in a Lake Victoria cyanobacterial bloom using LC–MS with thiol derivatization

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dc.contributor.author Miles, C. O.
dc.contributor.author Sandvik, H. E.
dc.contributor.author Rundberget, T.
dc.contributor.author Wilkins, A. L.
dc.contributor.author Rise, F.
dc.contributor.author Ballot, A.
dc.date.accessioned 2018-07-13T07:01:52Z
dc.date.available 2018-07-13T07:01:52Z
dc.date.issued 2013-04-06
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/2513
dc.description Toxicon, 2013; 70: 21-31 en_US
dc.description.abstract Microcystins are cyclic heptapeptides from cyanobacteria which are responsible for poisonings of livestock and humans. Cyanobacteria also produce a range of peptides and other compounds that can result in complex chromatograms when samples are analysed by LC– MS. Thiol derivatization of the a,b-unsaturated amide present in most microcystins was recently shown to simplify analysis of LC–MS chromatograms of a Microcystis culture, making it easier to identify peaks corresponding to microcystins in complex mixtures. This method was applied to analysis of extracts taken from a natural cyanobacteria bloom in Mwanza Gulf, Lake Victoria, Tanzania, in 2010, revealing the presence of numerous putative microcystin analogues in the sample. Results were verified using LC–MS2, LC–MS/MS with precursor-ion scanning, and LC–HRMS, leading to identification of 8 major and 17 minor microcystins in the sample, including analogues of microcystin-RY, -RL and -RA. Microcystin-YR (2), -RR (3), and -RY (9) were isolated from bloom material from Lake Victoria, and the structure of 9 was confirmed by NMR spectroscopic analysis and NMR spectral comparison with 2 and 3. Confirmation of the structure of MC-RY (9) facilitated detailed analysis of its MS2 spectrum, thereby supporting the structures of related analogues tentatively established on the basis of MS analyses en_US
dc.description.sponsorship The Norwegian Programme for Development, Research and Higher Education (NUFU PRO 07/10224) and SIDA SAREC: VICRES Endocrine disruptors project (SUA). en_US
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject Microcystin en_US
dc.subject Microcystis en_US
dc.subject Africa en_US
dc.subject Lake Victoria en_US
dc.subject LC–MS en_US
dc.subject Thiol derivatization en_US
dc.title Identification of microcystins in a Lake Victoria cyanobacterial bloom using LC–MS with thiol derivatization en_US
dc.type Article en_US
dc.url https://www.ncbi.nlm.nih.gov/pubmed/23567039 en_US


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