Identification of microcystins in a Lake Victoria cyanobacterial bloom using LC–MS with thiol derivatization

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dc.contributor.authorMiles, C. O.
dc.contributor.authorSandvik, H. E.
dc.contributor.authorRundberget, T.
dc.contributor.authorWilkins, A. L.
dc.contributor.authorRise, F.
dc.contributor.authorBallot, A.
dc.date.accessioned2018-07-13T07:01:52Z
dc.date.available2018-07-13T07:01:52Z
dc.date.issued2013-04-06
dc.descriptionToxicon, 2013; 70: 21-31en_US
dc.description.abstractMicrocystins are cyclic heptapeptides from cyanobacteria which are responsible for poisonings of livestock and humans. Cyanobacteria also produce a range of peptides and other compounds that can result in complex chromatograms when samples are analysed by LC– MS. Thiol derivatization of the a,b-unsaturated amide present in most microcystins was recently shown to simplify analysis of LC–MS chromatograms of a Microcystis culture, making it easier to identify peaks corresponding to microcystins in complex mixtures. This method was applied to analysis of extracts taken from a natural cyanobacteria bloom in Mwanza Gulf, Lake Victoria, Tanzania, in 2010, revealing the presence of numerous putative microcystin analogues in the sample. Results were verified using LC–MS2, LC–MS/MS with precursor-ion scanning, and LC–HRMS, leading to identification of 8 major and 17 minor microcystins in the sample, including analogues of microcystin-RY, -RL and -RA. Microcystin-YR (2), -RR (3), and -RY (9) were isolated from bloom material from Lake Victoria, and the structure of 9 was confirmed by NMR spectroscopic analysis and NMR spectral comparison with 2 and 3. Confirmation of the structure of MC-RY (9) facilitated detailed analysis of its MS2 spectrum, thereby supporting the structures of related analogues tentatively established on the basis of MS analysesen_US
dc.description.sponsorshipThe Norwegian Programme for Development, Research and Higher Education (NUFU PRO 07/10224) and SIDA SAREC: VICRES Endocrine disruptors project (SUA).en_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/2513
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectMicrocystinen_US
dc.subjectMicrocystisen_US
dc.subjectAfricaen_US
dc.subjectLake Victoriaen_US
dc.subjectLC–MSen_US
dc.subjectThiol derivatizationen_US
dc.titleIdentification of microcystins in a Lake Victoria cyanobacterial bloom using LC–MS with thiol derivatizationen_US
dc.typeArticleen_US
dc.urlhttps://www.ncbi.nlm.nih.gov/pubmed/23567039en_US

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