Roberto Luis NhamussuaFaith Philemone MabikiAlinanuswe Joel MwakalesiLyndy Joy McGaw2026-07-062026-07-062026-01Nhamussua RL, Mabiki FP, Mwakalesi AJ, McGaw LJ (2026) Screening anticancer activity by Brine shrimp lethality test of extracts of Annona stenophylla (Engl. & Diels), Strophanthus petersianus (Klotzsch) and Synadenium glaucescens (Pax). PLoS One 21(1): e0336636. https://doi.org/10.1371/ journal.pone.0336636https://www.suaire.sua.ac.tz/handle/20.500.14820/7678Journal ArticleCancer continues to be one of the main public health challenges, driving the search for new compounds with therapeutic potential. Medicinal plants represent a valuable promising source of bioactive metabolites, and the Brine Shrimp Lethality Test has been widely used as a preliminary tool to assess the toxicity of natural extracts, pro- viding clues to their possible anticancer activity. In this study, the cytotoxicity of the extracts of Annona stenophylla (Engl. & Diels), Strophanthus petersianus (Klotzsch), and Synadenium glaucescens (Pax) was investigated using the BSLT as a first step in screening for potential anticancer compounds. The plant materials were harvested in Tanzania and air-dried in the shade, and ground. The extracts were prepared by total sequential solvent extraction using cold maceration, starting with ethyl ace- tate, followed by methanol.enscreeninganticancercancertherapeutic potentialmedicinal plantsBrine Shrimp Lethality TestArticle