Sokoine University of Agriculture

Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species

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dc.contributor.author Ahmed, N.
dc.contributor.author Devi, S. M.
dc.contributor.author Valverde, M.
dc.contributor.author Vijayachari, P.
dc.contributor.author Machang'u, R. S.
dc.contributor.author Ellis, W. A.
dc.contributor.author Hartskeerl, R. A.
dc.date.accessioned 2017-06-16T15:24:36Z
dc.date.available 2017-06-16T15:24:36Z
dc.date.issued 2006-11-23
dc.identifier.uri https://www.suaire.sua.ac.tz/handle/123456789/1620
dc.description Annals of Clinical Microbiology and Antimicrobials 2006, Vol. 5:28 en_US
dc.description.abstract Leptospira are the parasitic bacterial organisms associated with a broad range of mammalian hosts and are responsible for severe cases of human Leptospirosis. The epidemiology of leptospirosis is complex and dynamic. Multiple serovars have been identified, each adapted to one or more animal hosts. Adaptation is a dynamic process that changes the spatial and temporal distribution of serovars and clinical manifestations in different hosts. Serotyping based on repertoire of surface antigens is an ambiguous and artificial system of classification of leptospiral agents. Molecular typing methods for the identification of pathogenic leptospires up to individual genome species level have been highly sought after since the decipherment of whole genome sequences. Only a few resources exist for microbial genotypic data based on individual techniques such as Multiple Locus Sequence Typing (MLST), but unfortunately no such databases are existent for leptospires. We for the first time report development of a robust MLST method for genotyping of Leptospira. Genotyping based on DNA sequence identity of 4 housekeeping genes and 2 candidate genes was analyzed in a set of 120 strains including 41 reference strains representing different geographical areas and from different sources. Of the six selected genes, adk, icdA and secY were significantly more variable whereas the LipL32 and LipL41 coding genes and the rrs2 gene were moderately variable. The phylogenetic tree clustered the isolates according to the genome-based species. The main advantages of MLST over other typing methods for leptospires include reproducibility, robustness, consistency and portability. The genetic relatedness of the leptospires can be better studied by the MLST approach and can be used for molecular epidemiological and evolutionary studies and population genetics. en_US
dc.language.iso en en_US
dc.subject Genotypic classification en_US
dc.subject Pathogenic Leptospira en_US
dc.subject Mammalian hosts en_US
dc.subject MLST en_US
dc.subject Clinical manifestations en_US
dc.title Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species en_US
dc.type Article en_US


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