Sokoine University of Agriculture

Genetic diversity among nine free range local chicken ecotypes in Tanzania based on microsatelliteDNA polymorphisms

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dc.contributor.author Msoffe, P. L. M.
dc.contributor.author Mtambo, M. M. A.
dc.contributor.author Minga, U. M.
dc.contributor.author juul-Madsen, H. R.
dc.contributor.author Gwakisa, P. S.
dc.date.accessioned 2017-04-13T07:37:21Z
dc.date.available 2017-04-13T07:37:21Z
dc.date.issued 2008-06
dc.identifier.uri https://www.suaire.sua.ac.tz/handle/123456789/1419
dc.description Tanzania Journal of Agricultural Sciences 2008, Vol. 9(1) : 54 - 68 en_US
dc.description.abstract The genetic diversity among nine free-range local chicken ecoivpes identified in Tanzania was evaluated using 20 polymorphic microsatelliies markers. The ecotypes were named Ching'toekuie, Mbeya, Morogoro-medium, Kuchi, Singamagazi, N'zenzegere, Pemba, Tunga, and Unguja. A manual microsatellite typing procedure was adopted. Of the 20 microsaielliie loci, 16 were amplified by PCR and were highly polymorphic. Four to 15 alleles per locus and four to seven alleles per ecotype were detected. Gene diversity was venj high (62 to 79%). The shortest genetic distance as measured by Nei's standard genetic distance (Ds) was between Kuchi and Unguja ecotypes (Ds distance = 0.0671) while the White Leghorn and Unguja ecotype (Ds distance= 0.9149) presented the longest genetic distance. A Neighbour-loining dendogram based on Ds distance showed the local chicken ecotypes to assort into three clusters. The bootstrapping values within the dendogram ranged from 41 to 98%. These results indicate that the free-range local chicken ecotypes to exist in different genetic groups. The accurate genetic typing of this animal genetic resource is important in making rational decisions on the utilisation of the chickens for economical use without compromising the conservation of each unique ecotype adapted to a particular eco-climaiic zone. en_US
dc.language.iso en en_US
dc.subject Genetic diversity en_US
dc.subject Free-range local chicken ecotypes en_US
dc.subject Microsatellites en_US
dc.subject Animal genetic resourcesIntroduction en_US
dc.title Genetic diversity among nine free range local chicken ecotypes in Tanzania based on microsatelliteDNA polymorphisms en_US
dc.type Article en_US


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