Sokoine University of Agriculture

Pcr detection of Leptospira DNA in rodents and insecti- vores from Tanzania

Show simple item record Mgode, G.F. Mhamphi, G. Katakweba, A. Paemelaere, E. Willekens, N. Leirs, H. Machang, R.S. Hartskeerl, R.A. 2016-11-22T08:36:17Z 2016-11-22T08:36:17Z 2005-12
dc.description Belgian journal of zoology 2005; 135 (supplement): 17-19 en_US
dc.description.abstract The true prevalence of leptospirosis in Tanzania is unknown or underestimated. In this study we report on the prevalence of leptospirosis in Morogoro, Tanzania, by PCR detection of leptospiral DNA in 27 kid- neys of rodents (Mastomys spp, Rattus spp, and Mus spp) and insectivores (Crocidura spp). The PCR study comple- mented previous attempts to isolate the leptospires and to perform seroprevalence by the microscopic agglutination test (MAT). Results of this study indicated an overall detection rate of 11 % by PCR, 7.4% by isolation and 0% by the MAT. Based on our analysis, it is recommended to use PCR and isolation for the detection of leptospires in potential host animals. en_US
dc.language.iso en en_US
dc.publisher Sokoine University of Agriculture en_US
dc.subject Leptospirosis en_US
dc.subject Prevalence en_US
dc.subject Microagglutination en_US
dc.subject Rodents en_US
dc.subject Tanzania en_US
dc.title Pcr detection of Leptospira DNA in rodents and insecti- vores from Tanzania en_US
dc.type Article en_US

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