Sokoine University of Agriculture

Quantification of markers of antimalarial drug resistance from an area of high malaria transmission: Comparing frequency with prevalence

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dc.contributor.author Malisa, Allen L.
dc.contributor.author Pearce, Richard J.
dc.contributor.author Mutayoba, Ben
dc.contributor.author Abdullah, Salim
dc.contributor.author Mshinda, Hassan
dc.contributor.author Kachur, Patrick
dc.contributor.author Bloland, Peter
dc.contributor.author Roper, Cally
dc.date.accessioned 2016-11-09T11:21:31Z
dc.date.available 2016-11-09T11:21:31Z
dc.date.issued 2012-08-28
dc.identifier.issn 1684–5315
dc.identifier.uri http://hdl.handle.net/123456789/882
dc.description African Journal of Biotechnology Vol. 11(69), pp. 13250-13260, 28 August, 2012 en_US
dc.description.abstract Molecular monitoring of markers of antimalarial drug resistance offers an affordable alternative to the in vivo method for the detection of resistance, and has the potential to guide public health policy in a timely manner. However, the optimal way of analyzing and reporting these data, particularly those emanating from areas of moderate to high malaria transmission, has never been fully explored or agreed upon, given the potential of being confounded by coinfections. By using large number of real field samples, we quantified the difference between prevalence and frequency when reporting field data on antimalarial drug resistance obtained by direct counting of haplotypes. Polymerase chain reaction (PCR) and sequence specific oligonucleotide probing was used to generate point mutations which were used to construct haplotypes. Results indicate that frequency underestimates haplotypes present at low levels while also amplifying haplotypes present at high levels; prevalence on the other hand behaved in a vice versa manner. Both prevalence and frequency are therefore essential, as each may have relevance in different contexts in high malaria transmission settings. Frequency is essential to gauge the impact of intervention on antimalarial drug resistance while prevalence may be more relevant when the aim is to determine parasite clearance en_US
dc.language.iso en en_US
dc.publisher Academic Journals en_US
dc.subject Molecular markers en_US
dc.subject Polymerase chain reaction (PCR) en_US
dc.subject Oligonucleotide probing en_US
dc.subject Prevalence en_US
dc.subject sequence specific oligonucleotide probing (SSOP) en_US
dc.subject Comparing frequency. en_US
dc.title Quantification of markers of antimalarial drug resistance from an area of high malaria transmission: Comparing frequency with prevalence en_US
dc.type Article en_US
dc.url http://www.academicjournals.org/AJB en_US


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