Sokoine University of Agriculture

Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania

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dc.contributor.author Misinzo, G.
dc.contributor.author Magambo, J.
dc.contributor.author Masambu, J.
dc.contributor.author Yongolo, M. G.
dc.contributor.author Doorsselaere, J. V.
dc.contributor.author Nauwynck, H. J.
dc.date.accessioned 2017-06-16T16:31:27Z
dc.date.available 2017-06-16T16:31:27Z
dc.date.issued 2011-02
dc.identifier.uri http://www.suaire.suanet.ac.tz:8080/xmlui/handle/123456789/1623
dc.description Transboundary and Emerging Diseases 2011Vol. 58(1): 86–92 en_US
dc.description.abstract Outbreaks of African swine fever (ASF) have been reported in the past from several countries in sub-Saharan Africa. The aim of this study was to genotype ASF viruses (ASFVs) from the 2008 outbreak in Morogoro and Dar es Salaam regions of Tanzania. Tissue samples from domestic pigs that died as a result of severe haemorrhagic disease were collected and analysed with PCR and genome sequencing methods using ASFV-specific primer sets. Nucleotide sequence data were obtained for the B646L (p72), E183L (p54) and the variable region of the B602L gene sequences. Phylogenetic analyses based on DNA sequences showed that the 2008 Tanzanian isolates belonged to p72 genotype XV and clustered together with those derived from the 2001 outbreak in Tanzania. Analysis of the tetrameric amino acid repeat regions within the variable region of the B602L gene showed that the repeat signature of the 2008 Tanzanian ASFV was unique and contained three novel tetramers (U = NIDT/NTDT and X = NTDI). Epidemiological investigation suggested that transportation of live pigs continues to play an active role in the epidemiology of ASF in Tanzania. It is recommended that future control of ASF spread in Tanzania should focus on the early detection and confirmation of the disease, prompt institution of quarantine measures, culling and proper disposal of infected and in-contact animals and decontamination of affected premises. en_US
dc.language.iso en en_US
dc.subject African swine fever outbreaks en_US
dc.subject Asfarviridae en_US
dc.subject Arbovirus en_US
dc.subject Domestic pigs en_US
dc.subject Genotyping en_US
dc.subject Phylogenetic analysis en_US
dc.title Genetic characterization of African swine fever viruses from a 2008 outbreak in Tanzania en_US
dc.type Article en_US
dc.url http://onlinelibrary.wiley.com/doi/10.1111/j.1865-1682.2010.01177.x/full en_US


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