Detection of serum neutralizing antibodies to Simbu sero-group viruses in cattle in Tanzania.

Simple item page
dc.contributor.authorMathew, C
dc.contributor.authorKlevar, S
dc.contributor.authorElbers, A
dc.contributor.authorvan der Poel, W
dc.contributor.authorKirkland, P
dc.contributor.authorGodfroid, J
dc.contributor.authorMdegela, R
dc.contributor.authorMwamengele, G
dc.contributor.authorStokstad, M
dc.date.accessioned2021-05-11T12:44:18Z
dc.date.available2021-05-11T12:44:18Z
dc.date.issued2015
dc.descriptionJournal articleen_US
dc.description.abstractBackground: Orthobunyaviruses belonging to the Simbu sero-group occur worldwide, including the newly recognized Schmallenberg virus (SBV) in Europe. These viruses cause congenital malformations and reproductive losses in ruminants. Information on the presence of these viruses in Africa is scarce and the origin of SBV is unknown. The aim of this study was to investigate the presence of antibodies against SBV and closely related viruses in cattle in Tanzania, and their possible association with reproductive disorders. Results: In a cross-sectional study, serum from 659 cattle from 202 herds collected in 2012/2013 were analyzed using a commercial kit for SBV ELISA, and 61 % were positive. Univariable logistic regression revealed significant association between ELISA seropositivity and reproductive disorders (OR = 1.9). Sera from the same area collected in 2008/2009, before the SBV epidemic in Europe, were also tested and 71 (54.6 %) of 130 were positive. To interpret the ELISA results, SBV virus neutralization test (VNT) was performed on 110 sera collected in 2012/2013, of which 51 % were positive. Of 71 sera from 2008/2009, 21 % were positive. To investigate potential cross reactivity with related viruses, 45 sera from 2012/2013 that were positive in SBV ELISA were analyzed in VNTs for Aino, Akabane, Douglas, Peaton, Sabo, SBV, Sathuperi, Shamonda, Simbu and Tinaroo viruses. All 45 sera were positive for one or more of these viruses. Twenty-nine sera (64.4 %) were positive for SBV, and one had the highest titer for this virus. Conclusions: This is the first indication that Aino, Akabane, Douglas, Peaton, Sabo, SBV, Sathuperi, Shamonda and Tinaroo viruses circulate and cause negative effect on reproductive performance in cattle in Tanzania. SBV or a closely related virus was present before the European epidemic. However, potential cross reactivity complicates the interpretation of serological studies in areas where several related viruses may circulate. Virus isolation and molecular characterization in cattle and/or vectors is recommended to further identify the viruses circulating in this region. However, isolation in cattle is difficult due to short viremic period of 2 to 6 days, and isolation in vectors does not necessarily reflect the situation in cattle.en_US
dc.description.sponsorshipEPINAV and PANTIL projectsen_US
dc.identifier.otherDOI 10.1186/s12917-015-0526-2
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/3516
dc.language.isoenen_US
dc.publisherBMC Veterinary Researchen_US
dc.subjectAntibody ELISAen_US
dc.subjectOrthobunya virusen_US
dc.subjectSerologyen_US
dc.subjectSchmallenberg virusen_US
dc.subjectVirus neutralizing testen_US
dc.titleDetection of serum neutralizing antibodies to Simbu sero-group viruses in cattle in Tanzania.en_US
dc.typeArticleen_US
dc.urlhttps://link.springer.com/article/10.1186/s12917-015-0526-2en_US

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Paper 3.pdf
Size:
473.07 KB
Format:
Adobe Portable Document Format
Description:
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.67 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Articles, Conferences and Workshop Papers Collection