Serological and molecular evidence of brucella species in the rapidly growing pig sector in Kenya

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dc.contributor.authorAkoko, J
dc.contributor.authorPelle, R
dc.contributor.authorKivali, V
dc.contributor.authorSchelling, E
dc.contributor.authorShirima, G
dc.contributor.authorMathew, C
dc.contributor.authorKyallo, V
dc.contributor.authorBonfoh, B
dc.contributor.authorKazwala, R
dc.contributor.authorOuma, C
dc.contributor.authorMachuka, E. M.
dc.contributor.authorFèvre, E. M.
dc.contributor.authorFalzon, L. C.
dc.contributor.authorLukambagire, A. S.
dc.contributor.authorHalliday, J. E. B.
dc.date.accessioned2021-05-11T12:51:00Z
dc.date.available2021-05-11T12:51:00Z
dc.date.issued2020
dc.descriptionJournal articleen_US
dc.description.abstractBackground: Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results: A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RTPCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion: The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs.en_US
dc.description.sponsorshipsupported within the framework of the DELTAS Africa Initiative [Afrique One-ASPIRE /DEL-15-008]. The Medical Research Council (UK) supported data collection and serological analysis, Biotechnology and Biological Science Research Council (UK), the Economic and Social Research Council (UK), the Natural Environment Research Council (UK), through the Environmental & Social Ecology of Human Infectious Diseases Initiative (ESEI), Grant Reference: G1100783/1 and the CGIAR Research Program on Agriculture for Nutrition and Health (A4NH), led by the International Food Policy Research Institute (IFPRI); we acknowledge the CGIAR Fund Donors (https:// www.cgiar.org/funders/). The molecular assays were conducted at the BecAILRI Hub through the Africa Biosciences Challenge Fund (ABCF) fellowship. The ABCF program is funded by the Australian Department for Foreign Affairs and Trade (DFAT) through the BecA-CSIRO partnership; the Syngenta Foundation for Sustainable Agriculture (SFSA); the Bill & Melinda Gates Foundation (BMGF); the UK Department for International Development (DFID) and the Swedish International Development Co-operation Agency (Sida).en_US
dc.identifier.otherdoi.org/10.1186/s12917-020-02346-y
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/3518
dc.language.isoenen_US
dc.publisherBMC Veterinary Researchen_US
dc.subjectPig brucellosisen_US
dc.subjectMolecular detectionen_US
dc.subjectMolecular evidenceen_US
dc.subjectBrucellaen_US
dc.subjectSerologyen_US
dc.subjectKenyaen_US
dc.titleSerological and molecular evidence of brucella species in the rapidly growing pig sector in Kenyaen_US
dc.typeArticleen_US
dc.urlhttps://pubmed.ncbi.nlm.nih.gov/32393374/en_US

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