Arbovirus infections in aedes mosquitoes at the interface of human-livestock-wildlife ecosystem in Kilosa district, Tanzania
| dc.contributor.author | Machelle, I. S | |
| dc.date.accessioned | 2021-07-08T18:09:26Z | |
| dc.date.available | 2021-07-08T18:09:26Z | |
| dc.date.issued | 2020 | |
| dc.description | Masters Thesis | en_US |
| dc.description.abstract | Arboviruses refer to a group of viruses, which are transmitted by arthropods including mosquitoes and ticks. The objective of this study was to determine mosquito transmission potential of arboviruses at the interface of human-livestock-wildlife ecosystem in Kilosa district, Tanzania. Adult mosquitoes were collected using Mosquito Magnet® Liberty Plus traps. Reverse transcription polymerase chain reaction assay was performed on pooled adult Aedes mosquitoes to detect the presence of Dengue virus (DENV), Chikungunya virus (CHIKV), Rift Valley fever virus (RVFV) and Yellow fever virus (YFV). A total of 1340 mosquitoes belonging to four genera (Aedes, Anopheles, Culex and Mansonia) and 6 species were collected. Culex accounted for the largest (48.06%; n= 644) proportion of the mosquitoes while Anopheles for the lowest proportion (2.54%; n=34). Of the total mosquitoes collected, Aedes aegypti accounted for the majority of mosquito species (46%; n=613), followed by Culex quinquefasciatus (44.1%; n=591). Of the 36 Ae. aegypti pools tested for arbovirus 10 (28%) pools were positive. Dengue virus was detected in 3% (1/ 36) pools and CHIKV in 25% (9/ 36) pools. One pool that was positive for DENV also tested positive for CHIKV indicating the possibility of co-infection whereby, individuals may become infected by more than one arbovirus at a time thus risk of co-transmission to human and livestock. Nucleotide sequencing of polymerase chain reaction (PCR) products of the structural polyprotein region of DENV produced 511 bp fragment. Basic Local Alignment Search tool for nucleotides (BLASTn) and phylogenetic analysis showed that the (DENV3/TAN/Mikumi/2020 strain) (Accession number MW133786) obtained from this study clustered with DENV-3 strains reported in China and Kenya. This information is important as it gives knowledge on areas at high risk for arboviral disease outbreaks. The findings indicate that the presence of various mosquito vectors and detection of arboviruses in wild-caught Aedes mosquitoes leave the population of Kilosa district at a higher risk of transmission of DENV and CHIKV | en_US |
| dc.description.sponsorship | SACIDS | en_US |
| dc.identifier.uri | https://www.suaire.sua.ac.tz/handle/123456789/3674 | |
| dc.language.iso | en | en_US |
| dc.publisher | Sokoine University of Agriculture | en_US |
| dc.subject | Arbovirus infections | en_US |
| dc.subject | Aedes mosquitoes | en_US |
| dc.subject | Human-livestock-wildlife | en_US |
| dc.subject | Kilosa | en_US |
| dc.subject | Tanzania | en_US |
| dc.title | Arbovirus infections in aedes mosquitoes at the interface of human-livestock-wildlife ecosystem in Kilosa district, Tanzania | en_US |
| dc.type | Thesis | en_US |