Monitoring proteolysis in milk caused by pseudomonas fluorescens by the TNBS method

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dc.contributor.authorChove, L. M
dc.contributor.authorGrandison, A.
dc.contributor.authorLewis, M.
dc.date.accessioned2023-03-06T10:41:59Z
dc.date.available2023-03-06T10:41:59Z
dc.date.issued2017-11
dc.descriptionJournal articleen_US
dc.description.abstractThe objective of this study was to monitor proteolysis of milk during storage at 20 and 4° C by two strains of Pseudomonas fluorescens namely: NCIMB 702085 (416) and NCIMB 701274 (414) by the TNBS method. Since commercial Pseudomonas fluorescens enzymes are not available, live Pseudomonas fluorescens microorganisms were inoculated into milk followed by extraction and purification by dialysis. Bacterial enzymes obtained were then inoculated into UHT skim milk. Non-inoculated milk was used as a control. Results obtained showed that Pseudomonas fluorescens NCIMB 702085 was more proteolytic than Pseudomonas fluorescens NCIMB 701274. It was concluded that the TNBS method proved useful in monitoring proteolysis in milk by Pseudomonas fluorescens.en_US
dc.identifier.issn2465-7522
dc.identifier.urihttp://www.suaire.sua.ac.tz/handle/123456789/4982
dc.language.isoenen_US
dc.publisherPearl Research Journalsen_US
dc.subjectBacterial enzymesen_US
dc.subjectDialysisen_US
dc.subjectPurificationen_US
dc.subjectUHT skim milken_US
dc.titleMonitoring proteolysis in milk caused by pseudomonas fluorescens by the TNBS methoden_US
dc.typeArticleen_US
dc.urlhttp://pearlresearchjournals.org/journals/jasft/index.htmlen_US

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