Validation of droplet digital Polymerase Chain Reaction for the detection and absolute quantification of Taenia solium eggs in spiked soil samples

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dc.contributor.authorMaganira, Justine Daudi
dc.contributor.authorMwang'onde, Beda John
dc.contributor.authorKidima, Winifrida
dc.contributor.authorMwita, Chacha John
dc.contributor.authorNkwengulila, Gamba
dc.contributor.authorHöglund, Johan
dc.date.accessioned2021-08-06T05:16:31Z
dc.date.available2021-08-06T05:16:31Z
dc.date.issued2019
dc.descriptionJournal articleen_US
dc.description.abstractTo enable the detection of taeniid eggs in environmental samples, a sensitive technology is required. In this study, we validated the effectiveness of a digital droplet Polymerase Chain Reaction (ddPCR) assay for detection, identification and absolute quantification of taeniid DNA from artificially contaminated soils with varying numbers of taeniid eggs using a set of universal primers, JB3 & JB4.5. The results showed that the number of cox1 copies detected increased gradually for both species with the number of taeniid eggs added to the different soil types. The highest cox1 DNA copies recovery for Taenia solium and T. lynciscapreoli was from the sand soil with lowest recovery being observed in clay soils. Therefore, ddPCR is a promising technology for screening of taeniid eggs from soil samples collected in the environment irrespective of the soil type and the number of eggs. The potential of the ddPCR protocol to detect taeniid egg DNA in spiked soil samples has great practical application for taeniid egg screening in soils from endemic areas. However, when universal primers are used in screening environmental samples, the identity of ddPCR positive samples must be confirmed by sequencing. In addition, more validation studies using species-specific primers and field soil samples is recommended.en_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/3831
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectSoilen_US
dc.subjectddPCRen_US
dc.subjectTaeniid eggsen_US
dc.subjectTaenia soliumen_US
dc.subjectTaenia lynciscapreolien_US
dc.subjectDetectionen_US
dc.titleValidation of droplet digital Polymerase Chain Reaction for the detection and absolute quantification of Taenia solium eggs in spiked soil samplesen_US
dc.typeArticleen_US
dc.urlhttps://doi.org/10.1016/j.actatropica.2019.105175en_US

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