Comparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganisms

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dc.contributor.authorGidamis, A.B.
dc.contributor.authorNnko, S.A.
dc.contributor.authorShayo, N.B.
dc.contributor.authorChove, B.E.
dc.contributor.authorKroner, H. K.
dc.date.accessioned2017-12-08T11:27:35Z
dc.date.available2017-12-08T11:27:35Z
dc.date.issued1998
dc.descriptionTanzania Journal of Agriculture Science 1998, Vol. 1(2), pp 141-148en_US
dc.description.abstractA stlldy was conducted to compare the efficiency of two methods of microbial cell separation and enzyme purification using penicillin-G-acylase (PGA) from Escherichia coli. The efficiency of two methods of ceJ[ separation; centrifugation and cross flow filtration (CFF) were compared. The CFF method was found to have both higher separation efficiency and enzyme yield than centrifugation method. Centrifugation method gave a separation efficiency of 98.5% with enzyme yield of94 % whereas CFFmethod resulted in 100% separation efficiency and enzyme yield of98.8%. The Escherichia coli cells were disrupted by high pressure homogenization (HPH),and the disrupted cells were purified using two different techniques. Technique I was a combination of cross-flow-diafiltration (CFD) , ultrafiltration (UF) and heat/pH-shift treatments. This technique resulted in 47% enzyme yield with a purification factor of 12. Technique II which involved two extraction steps by' aqueous two - phase system (APS) coupled with UF resulted in 62 % enzyme yield with a pu;ificationfactor of 4. Technique I was therefore much better than techriique Il in purifying the enzyme. For higher enzyme yield, technique II would seem to be a better one than technique I.en_US
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/1845
dc.language.isoenen_US
dc.subjectMicrobial enzymesen_US
dc.subjectPurification factoren_US
dc.subjectRecovery rateen_US
dc.subjectSeparation efficiencyen_US
dc.subjectTechnical enzymeen_US
dc.titleComparative evaluation of different methods of extraction and purification used in technical enzyme production from microorganismsen_US
dc.typeArticleen_US

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