Filter paper is a simple and cost-effective transport medium for serological diagnosis of Peste des petits ruminants

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dc.contributor.authorTorsson, Emeli
dc.contributor.authorAbubakar, Muhammad
dc.contributor.authorAlvÄsen, Karin
dc.contributor.authorManzoor, Shumaila
dc.contributor.authorRoos, Nils
dc.contributor.authorZahur, Aamer
dc.contributor.authorMisinzo, Gerald
dc.contributor.authorBerg, Mikael
dc.contributor.authorWensman, Jonas
dc.date.accessioned2021-11-23T07:50:48Z
dc.date.available2021-11-23T07:50:48Z
dc.date.issued2019-01
dc.description.abstractPeste des petits ruminants (PPR) is a highly contagious disease caused by peste-des-petits-ruminants virus. Following the successful eradication of the related rinderpest virus, a program to control and eradicate PPR was launched by the FAO and OIE. PPR is today present in many tropical countries where maintaining the cold chain for sample transportation is one of the major barriers for timely processing. Transport of samples on filter paper is a simple and cost-effective method, however validation and optimization is required to fully adapt this approach. The objective of this study was to evaluate and validate the use of filter paper in serological diagnosis of PPR. Blood samples (serum and filter paper) were collected from sheep and goats in both Tanzania and Pakistan and analysed using a PPRV-specific cELISA. The positive proportion was 10.7% in Tanzania and 80% in Pakistan when performing the analysis on serum. These results were then considered as reference and compared to the results from the filter papers analysed by the same cELISA. According to the statistical analysis the cut-off for a positive results for samples stored on filter paper was adjusted from <50 % competition percentage to <84% in Tanzania and to <69% in Pakistan. These results demonstrate that filter papers are an acceptable and cost-effective transport method of whole blood samples for later use in serological analysis.en_US
dc.identifier.issn0921-4488
dc.identifier.urihttps://www.suaire.sua.ac.tz/handle/123456789/3903
dc.language.isoenen_US
dc.publisherElsevieen_US
dc.subjectPeste des petits ruminantsen_US
dc.subjectserological diagnosisen_US
dc.subject, filter papersen_US
dc.subjecttransporten_US
dc.subjectTanzaniaen_US
dc.subjectPakistanen_US
dc.titleFilter paper is a simple and cost-effective transport medium for serological diagnosis of Peste des petits ruminantsen_US
dc.typeArticleen_US
dc.urlhttps://doi.org/10.1016/j.smallrumres.2018.11.023en_US

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